Publikationsdatenbank

Histomorphological development of the jejunum in low and normal birth weight piglets orally supplemented with glutamine during the suckling period (2021)

Art

Poster

Autoren

Schulze Holthausen, J. (WE 4)

Sciascia, Q. L.

Schregel, J.

Li, Z.

Metges, C. C.

Zentek, J. (WE 4)

Kongress

75. digitale Tagung der Gesellschaft für Ernährungsphysiologie

Berlin, 16. – 18.03.2021

Quelle

75th digital conference 16th-18th March 2021 = 75. digitale Tagung vom 16.-18. März 2021 — editor: Society of Nutrition Physiology ; Redaktion: D. Kampf, S. Ausmeier, S. Trappe (Gesellschaft für Ernährungsphysiologie) (Hrsg.)

Frankfurt am Main (Germany): DLG-Verlag GmbH, 2021. Proceedings of the Society of Nutrition Physiology : Berichte der Gesellschaft für Ernährungsphysiologie ; volume 30 (2021) — S. 63

ISBN: 978-3-7690-4114-9

Sprache

Englisch

Verweise

URL (Volltext): http://d-nb.info/1229112979

Kontakt

Institut für Tierernährung

Königin-Luise-Str. 49
14195 Berlin
+49 30 838 52256
tierernaehrung@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Improved sow breeding techniques have led to increased numbers of low birth weight (LBW) piglets. The jejunum plays an important role in nutrient absorption and innate immune defense (1) and its development and function may be impaired in LBW piglets (2). Supplemental glutamine (GLN) has been shown to improve intestinal structure in weaning and post-weaning piglets (3); however, the influence of GLN during the early neonatal period has not been assessed. We hypothesize that GLN improves jejunal development and morphology in suckling LBW piglets. Methods: At birth, pairs of LBW (0.8 - 1.2 kg) and normal (NBW, 1.4 - 1.8 kg) male littermates born to gilts were selected. Approximately 24 hours after birth, litters were standardised to 12 piglets and experimental piglets were assigned to one of two isonitrogenous supplementation groups: GLN = 1 g/kg bodyweight; or alanine (ALA) = 1.22 g/kg. Thus, four different groups were studied: (LBW+GLN; NBW+GLN; LBW+ALA; NBW+ALA) (n = 12 / group). Piglets were orally supplemented 3 x daily (7:00, 12:00, 17:00) until d 12. Piglets were suckled by their respective dam for the duration of the study and had access to creep feed from d 14. Subgroups were euthanized at d 5, 12 and 26 (n = 48 / time point) and a section of mid-jejunal tissue was fixed in formalin. Histological examination of tissue morphology and neutral, acidic and mixed mucins was performed by Alcian blue pH 2.5 -periodic acid Schiff staining and images examined with the cellSens imaging software. Data were analyzed by multivariate ANOVA followed by Tukey post hoc test. Results: Age comparisons showed for all supplementation groups villus height (VH) increased from d 5 to 12 and decreased from d 12 to 26 (P < 0.01), crypt depth (CD) increased from d 5 to 12, and 12 to 26 (P < 0.01). The VH:CD ratio decreased from d 5 to 12, and d 12 to 26 for all groups except ALA+NBW which increased from d 5 to 12 (P < 0.05), and decreased from 12 to 26 (P < 0.01). The total number of goblet cells in villi and crypts decreased from d 5 to 12 (P < 0.01) and increased from d 12 to 26 (P < 0.01). Supplementation and birth weight had no effect on the investigated parameters. Conclusion: The results show that LBW may not be associated with impaired jejunal morphometric development compared to NBW littermates, and that GLN supplementation has no observable effect on the measured parameters. Age appears to have the most significant impact independent of supplementation and birth weight.

(1) FERENEC et al. (2017): Dis. Markers: 5238134
(2) D’INCA et al. J. Nutr. 133: 1-3
(3) WU et al. J. Nutr. 126: 2578-2584