Publication Database

Efficacy of CpG-ODN inhalation therapy versus a combined formulation of fluticasone and salmeterol für the therapy of equine asthma (2021)

Art

Poster

Autoren

Barton, Ann Kristin (WE 17)

Ladendorf, Ina (WE 17)

Lehmann, Beatrice (WE 17)

Schmitz, Philip (WE 17)

Merle, Roswitha (WE 16)

Gehlen, Heidrun (WE 17)

Kongress

ECEIM Jahreskongress

online, 24. – 25.10.2021

Quelle

Sprache

Englisch

Verweise

URL (Volltext): https://www.eceim.info/system/files/poster-presentations/2021/poster-presentation-798.pdf

Kontakt

Institut für Veterinär-Epidemiologie und Biometrie

Königsweg 67
14163 Berlin
+49 30 838 56034
epi@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Introduction:
Inhalation of immunostimulatory bacterial DNA segments (cytosine-phosphate-guanosine-oligodeoxynucleotides, CpG-ODN) has been shown to normalize clinical and cytologic parameters in severe equine asthma. In this study, we evaluated the efficacy of CpG-ODNs in comparison to a combined formulation of fluticasone/salmeterol and placebo in asthmatic horses. Several medical trials with human asthma patients have indicated that a combination product containing both fluticasone and salmeterol is superior to the respective monotherapies.

Methods:
A modified clinical scoring including auscultatory and endoscopic findings, partial pressure of oxygen and percentage of neutrophil granulocytes in the bronchoalveolar lavage fluid (BALF) was used to include 33 horses (ø 15.2 years) of mixed gender and breed suffering from equine asthma. Horses received inhalation therapy twice daily for ten days with CpG-ODN (n=13), fluticasone/salmeterol (F-S, Serroflo™, Cipla Germany, n=10) or 0.9% saline (placebo, n=10) and were controlled afterwards.

Results:
Both CpG-ODN and F-S led to significant reductions (P < 0.05) in dyspnea and auscultatory findings compared to placebo. Only F-S reduced the overall clinical scoring, tracheal secretion, percentages of BALF neutrophils, while only CpG-ODN reduced respiratory rates. F-S efficacy was superior to CpG-ODN concerning the reduction of BALF neutrophils (P = 0.000) and overall clinical scoring (P = 0.003).

Discussion and clinical relevance:
F-S and CPG-ODN inhalation can be recommended for the treatment of equine asthma. In this study, the combined F-S inhalation was clearly more
effective than CpG-ODN therapy, although former studies have shown that the efficacy of CpG-ODNs is comparable and longer lasting than beclomethasone monotherapy (Klier et al. 2019). Therefore, the combined effect of CpG-ODN with bronchodilators should be studied in the future.