Publication Database

Specific target proteins and their role in angiogenesis in vitro (2021)

Art

Poster

Autoren

Herre, Christina (WE 1)

Nshdejan, Arpenik (WE 1)

Klopfleisch, Robert (WE 12)

Corte, Giuliano Mario (WE 1)

Bahramsoltani, Mahtab (WE 1)

Kongress

33rd Virtual Conference of the European Association of Veterinary Anatomists

Ghent, Belgium, 28. – 31.07.2021

Quelle

Anatomia, histologia, embryologia

Bandzählung: 51

Heftzählung: S1

Seiten: 28

ISSN: 0340-2096

Sprache

Englisch

Verweise

URL (Volltext): https://onlinelibrary.wiley.com/doi/full/10.1111/ahe.12759

DOI: 10.1111/ahe.12759

Kontakt

Institut für Veterinär-Anatomie

Koserstr. 20
14195 Berlin
+49 30 838 75784
anatomie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Introduction:
Angiogenesis plays a significant role in ischemic diseases, tumor growth and metastasis. Still in vitro assays of angiogenesis frequently fail considering their reproducibility, potentially due to variances in the angiogenic potency of Endothelial Cells (ECs). Cellular mechanisms which increase or decrease the angiogenic potency are still obscure. During previous analyses, eight specific proteins were identified to be expressed differentially in angiogenic and non-angiogenic ECs (Bahramsoltani et al. Clin Hemorheol Microcirc 2013; 55, 255-269). This study focuses on three of these proteins, i.e. vimentin, adenosylmethionine synthetase isoform type-2 and triosephosphate isomerase, and their impact on the angiogenic potency.

Materials and Methods:
Human dermal microvascular ECs were cultivated in a proangiogenic medium. The process of angiogenesis in vitro was quantified by staging the time-dependent morphological changes. Vimentin’s influence on the angiogenic potency of the cultured ECs was examined by shRNA-mediated knock-down and subsequent quantification of angiogenesis. Protein- and mRNA-levels of all proteins were detected at four time points via Western blot and RT-qPCR.

Results:
Preliminary results show that native ECs were able to run through all stages of angiogenesis in vitro, chronologically. In contrast, infected cells were unable to enter late stages of angiogenesis. Furthermore, all proteins showed an increased expression in early stages of the angiogenic cascade, followed by a decrease.

Conclusion:
The expression levels of all three proteins appeared to be dependent on specific angiogenic stages. Gene silencing of vimentin inhibits ECs to enter all stages of angiogenesis in vitro, concluding that vimentin raises their angiogenic potency.