Publikationsdatenbank

Culture of bovine mammary gland (2021)

Art

Poster

Autoren

Bonack, Annekatrin (WE 1)

Al Masri, Salah (WE 1)

Plendl, Johanna (WE 1)

Kongress

33rd Virtual Conference of the European Association of Veterinary Anatomists

Ghent, Belgium, 28. – 31.07.2021

Quelle

Anatomia, histologia, embryologia

Bandzählung: 51

Heftzählung: S1

Seiten: 10 – 11

ISSN: 0340-2096

Sprache

Englisch

Verweise

URL (Volltext): https://onlinelibrary.wiley.com/doi/full/10.1111/ahe.12759

DOI: 10.1111/ahe.12759

Kontakt

Institut für Veterinär-Anatomie

Koserstr. 20
14195 Berlin
+49 30 838 75784
anatomie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Introduction:
Whilst in vitro production of meat is well advanced this is not the case for milk where, worldwide, only two companies currently produce milk from cultured mammary epithelial cells. The aim of this pilot study is to establish bovine mammary cell cultures to investigate their potential for in vitro milk production.

Material and Methods:
Samples of bovine mammary glandular parenchymal tissue were taken from both heifers and post-parturient cows and finely minced (0,5 – 1,0 mm³). Samples were cultured in both coated and non-coated 6-well culture plates with a selective medium for mammary epithelial cells (PromoCell), without fetal bovine serum but with growth factors including epidermal growth factor and bovine pituitary extract. Cultures were incubated at 37,5°C (5% CO2) and the medium was changed every 48 hours.

Results:
After two days, filamentous strands appeared that bridged peripheral irregularities such as fissures and discontinuities of the samples. After seven days single rounded or sprouting cells appeared in close proximity but without connection to the core samples. Occasionally net-like filamentous structures were found that included single rounded cells. From day twelve onward a homogenous population of rounded/polygonal cells created a cobblestone appearance out from the periphery of the samples. No contamination with fibroblast-like cells was seen.

Conclusion:
The selective and serum-free medium supported the growth of a homogenous population of cells. Formation of filamentous structures seemed to precede cellular proliferation. Further studies will identify the nature of the filamentous structures and cells.