Publikationsdatenbank

Cas9-expressing chickens and pigs as resources for genome editing in livestock (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Rieblinger, Beate

Sid, Hicham

Duda, Denise

Bozoglu, Tarik

Klinger, Romina

Schlickenrieder, Antonina

Lengyel, Kamila

Flisikowski, Krzysztof

Flisikowska, Tatiana

Simm, Nina

Grodziecki, Alessandro

Perleberg, Carolin

Bähr, Andrea

Carrier, Lucie

Kurome, Mayuko

Zakhartchenko, Valeri

Kessler, Barbara

Wolf, Eckhard

Kettler, Lutz

Luksch, Harald

Hagag, Ibrahim T. (WE 5)

Wise, Daniel

Kaufman, Jim

Kaufer, Benedikt B. (WE 5)

Kupatt, Christian

Schnieke, Angelika

Schusser, Benjamin

Quelle

Proceedings of the National Academy of Sciences of the United States of America

Bandzählung: 118

Heftzählung: 10

Seiten: Artikel e2022562118

ISSN: 1091-6490

Sprache

Englisch

Verweise

URL (Volltext): https://www.pnas.org/content/118/10/e2022562118

DOI: 10.1073/pnas.2022562118

Pubmed: 33658378

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Genetically modified animals continue to provide important insights into the molecular basis of health and disease. Research has focused mostly on genetically modified mice, although other species like pigs resemble the human physiology more closely. In addition, cross-species comparisons with phylogenetically distant species such as chickens provide powerful insights into fundamental biological and biomedical processes. One of the most versatile genetic methods applicable across species is CRISPR-Cas9. Here, we report the generation of transgenic chickens and pigs that constitutively express Cas9 in all organs. These animals are healthy and fertile. Functionality of Cas9 was confirmed in both species for a number of different target genes, for a variety of cell types and in vivo by targeted gene disruption in lymphocytes and the developing brain, and by precise excision of a 12.7-kb DNA fragment in the heart. The Cas9 transgenic animals will provide a powerful resource for in vivo genome editing for both agricultural and translational biomedical research, and will facilitate reverse genetics as well as cross-species comparisons.