Publikationsdatenbank

Impact of porcine cytomegalovirus on long-term orthotopic cardiac xenotransplant survival (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Denner, Joachim (WE 5)

Längin, Matthias

Reichart, Bruno

Krüger, Luise

Fiebig, Uwe

Mokelke, Maren

Radan, Julia

Mayr, Tanja

Milusev, Anastasia

Luther, Fabian

Sorvillo, Nicoletta

Rieben, Robert

Brenner, Paolo

Walz, Christoph

Wolf, Eckhard

Roshani, Berit

Stahl-Hennig, Christiane

Abicht, Jan-Michael

Quelle

Scientific reports

Bandzählung: 10

Seiten: Article number: 17531

ISSN: 2045-2322

Sprache

Englisch

Verweise

URL (Volltext): https://www.nature.com/articles/s41598-020-73150-9

DOI: 10.1038/s41598-020-73150-9

Pubmed: 33067513

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Xenotransplantation using pig organs has achieved survival times up to 195 days in pig orthotopic heart transplantation into baboons. Here we demonstrate that in addition to an improved immunosuppressive regimen, non-ischaemic preservation with continuous perfusion and control of post-transplantation growth of the transplant, prevention of transmission of the porcine cytomegalovirus (PCMV) plays an important role in achieving long survival times. For the first time we demonstrate that PCMV transmission in orthotopic pig heart xenotransplantation was associated with a reduced survival time of the transplant and increased levels of IL-6 and TNFα were found in the transplanted baboon. Furthermore, high levels of tPA-PAI-1 complexes were found, suggesting a complete loss of the pro-fibrinolytic properties of the endothelial cells. These data show that PCMV has an important impact on transplant survival and call for elimination of PCMV from donor pigs.