Publikationsdatenbank

Identification of cardiac malformations in mice lacking Ptdsr using a novel high-throughput magnetic resonance imaging technique (2004)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Schneider, Jürgen E.

Böse, Jens

Bamforth, Simon D.

Gruber, Achim D. (WE 12)

Broadbent, Carol

Clarke, Kieran

Neubauer, Stefan

Lengeling, Andreas

Bhattacharya, Shoumo

Quelle

BMC developmental biology

Bandzählung: 4

Seiten: 16

ISSN: 1471-213x

Sprache

Englisch

Verweise

URL (Volltext): https://bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-4-16

DOI: 10.1186/1471-213X-4-16

Pubmed: 15615595

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background: Congenital heart defects are the leading non-infectious cause of death in children. Genetic studies in the mouse have been crucial to uncover new genes and signaling pathways associated with heart development and congenital heart disease. The identification of murine models of congenital cardiac malformations in high-throughput mutagenesis screens and in gene-targeted models is hindered by the opacity of the mouse embryo.

Results: We developed and optimized a novel method for high-throughput multi-embryo magnetic resonance imaging (MRI). Using this approach we identified cardiac malformations in phosphatidylserine receptor (Ptdsr) deficient embryos. These included ventricular septal defects, double-outlet right ventricle, and hypoplasia of the pulmonary artery and thymus. These results indicate that Ptdsr plays a key role in cardiac development.

Conclusions: Our novel multi-embryo MRI technique enables high-throughput identification of murine models for human congenital cardiopulmonary malformations at high spatial resolution. The technique can be easily adapted for mouse mutagenesis screens and, thus provides an important new tool for identifying new mouse models for human congenital heart diseases.