Publication Database

Weigners fixative:
an alternative to formalin fixation for histology with improved preservation of nucleic acids (2013)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Klopfleisch, R. (WE 12)

von Deetzen, M. (WE 12)

Weiss, A. Th. (WE 12)

Weigner, J. (WE 1)

Weigner, F. (WE 12)

Plendl, J. (WE 1)

Gruber, A. D. (WE 12)

Quelle

Veterinary pathology : an internat. journal of natural and experimental disease in animals

Bandzählung: 50

Heftzählung: 1

Seiten: 191 – 199

ISSN: 0300-9858

Sprache

Englisch

Verweise

URL (Volltext): https://journals.sagepub.com/doi/10.1177/0300985812441031

DOI: 10.1177/0300985812441031

Pubmed: 22539409

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Formalin fixation and paraffin embedding (FFPE) is the standard method for tissue storage in histopathology. However, FFPE has disadvantages in terms of user health, environment, and nucleic acid integrity. Weigners fixative has been suggested as an alternative for embalming cadavers in human and veterinary anatomy. The present study tested the applicability of Weigners for histology and immunohistochemistry and the preservation of nucleic acids. To this end, a set of organs was fixed for 2 days and up to 6 months in Weigners (WFPE) or formalin. WFPE tissues from the skin, brain, lymphatic tissues, liver, and muscle had good morphologic preservation, comparable to formalin fixation. The quality of kidney and lung samples was inferior to FFPE material due to less accentuated nuclear staining and retention of proteinaceous interstitial fluids. Azan, Turnbull blue, toluidin, and immunohistochemical stainings for CD79a, cytokeratin, vimentin, and von Willebrand factor led to comparable results with both fixates. Of note, immunohistochemical detection of CD3 was possible after 6 months in WFPE but not in FFPE tissues. mRNA, miRNA, and DNA from WFPE tissues had superior quality and allowed for amplification of miRNA, 400-bp-long mRNA, and 1000-bp-long DNA fragments after 6 months of fixation in WFPE. In summary, Weigners fixative is a nonhazardous alternative to formalin, which provides a good morphologic preservation of most organs, a similar sensitivity for protein detection, and a superior preservation of nucleic acids. Weigners may therefore be a promising alternative to cryopreservation and may be embraced by people affected by formalin allergies.