Publikationsdatenbank

Temporal omics analysis in Syrian hamsters unravel cellular effector responses to moderate COVID-19 (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Nouailles, Geraldine

Wyler, Emanuel

Pennitz, Peter

Postmus, Dylan

Vladimirova, Daria (WE 5)

Kazmierski, Julia

Pott, Fabian

Dietert, Kristina (WE 12)

Muelleder, Michael

Farztdinov, Vadim

Obermayer, Benedikt

Wienhold, Sandra-Maria

Andreotti, Sandro

Hoefler, Thomas (WE 5)

Sawitzki, Birgit

Drosten, Christian

Sander, Leif E.

Suttorp, Norbert

Ralser, Markus

Beule, Dieter

Gruber, Achim D. (WE 12)

Goffinet, Christine

Landthaler, Markus

Trimpert, Jakob (WE 5)

Witzenrath, Martin

Quelle

Nature Communications

Bandzählung: 12

Heftzählung: 1

Seiten: Article number: 4869

ISSN: 2041-1723

Sprache

Englisch

Verweise

URL (Volltext): https://www.nature.com/articles/s41467-021-25030-7

DOI: 10.1038/s41467-021-25030-7

Pubmed: 34381043

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

In COVID-19, immune responses are key in determining disease severity. However, cellular mechanisms at the onset of inflammatory lung injury in SARS-CoV-2 infection, particularly involving endothelial cells, remain ill-defined. Using Syrian hamsters as a model for moderate COVID-19, we conduct a detailed longitudinal analysis of systemic and pulmonary cellular responses, and corroborate it with datasets from COVID-19 patients. Monocyte-derived macrophages in lungs exert the earliest and strongest transcriptional response to infection, including induction of pro-inflammatory genes, while epithelial cells show weak alterations. Without evidence for productive infection, endothelial cells react, depending on cell subtypes, by strong and early expression of anti-viral, pro-inflammatory, and T cell recruiting genes. Recruitment of cytotoxic T cells as well as emergence of IgM antibodies precede viral clearance at day 5 post infection. Investigating SARS-CoV-2 infected Syrian hamsters thus identifies cell type-specific effector functions, providing detailed insights into pathomechanisms of COVID-19 and informing therapeutic strategies.