Publikationsdatenbank

Lectin-mediated bacterial modulation by the intestinal nematode Ascaris suum (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Midha, Ankur (WE 6)

Goyette-Desjardins, Guillaume

Gordeler, Felix

Mocovitz, Oren

Seeberger, Peter H.

Tedin, Karsten (WE 7)

Bertzbach, Luca D. (WE 5)

Lepenies, Bernd

Hartmann, Susanne (WE 6)

Quelle

International journal of molecular sciences

Bandzählung: 22

Heftzählung: 16

Seiten: Artikel 8739

ISSN: 1422-0067

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/1422-0067/22/16/8739

DOI: 10.3390/ijms22168739

Pubmed: 34445445

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Ascariasis is a global health problem for humans and animals. Adult Ascaris nematodes are long-lived in the host intestine where they interact with host cells as well as members of the microbiota resulting in chronic infections. Nematode interactions with host cells and the microbial environment are prominently mediated by parasite-secreted proteins and peptides possessing immunomodulatory and antimicrobial activities. Previously, we discovered the C-type lectin protein AsCTL-42 in the secreted products of adult Ascaris worms. Here we tested recombinant AsCTL-42 for its ability to interact with bacterial and host cells. We found that AsCTL-42 lacks bactericidal activity but neutralized bacterial cells without killing them. Treatment of bacterial cells with AsCTL-42 reduced invasion of intestinal epithelial cells by Salmonella. Furthermore, AsCTL-42 interacted with host myeloid C-type lectin receptors. Thus, AsCTL-42 is a parasite protein involved in the triad relationship between Ascaris, host cells, and the microbiota.