Publikationsdatenbank

Presence of β-lactamase-producing enterobacterales and Salmonella isolates in marine mammals (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Grünzweil, Olivia M.

Palmer, Lauren

Cabal, Adriana

Szostak, Michael P.

Ruppitsch, Werner

Kornschober, Christian

Korus, Maciej

Misic, Dusan

Bernreiter-Hofer, Tanja

Korath, Anna D. J.

Feßler, Andrea T. (WE 7)

Allerberger, Franz

Schwarz, Stefan (WE 7)

Spergser, Joachim

Müller, Elke

Braun, Sascha D.

Monecke, Stefan

Ehricht, Ralf

Walzer, Chris

Smodlaka, Hrvoje

Loncaric, Igor

Quelle

International journal of molecular sciences

Bandzählung: 22

Heftzählung: 11

Seiten: Artikel 5905

ISSN: 1422-0067

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/1422-0067/22/11/5905

DOI: 10.3390/ijms22115905

Pubmed: 34072783

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Marine mammals have been described as sentinels of the health of marine ecosystems. Therefore, the aim of this study was to investigate (i) the presence of extended-spectrum β-lactamase (ESBL)- and AmpC-producing Enterobacterales, which comprise several bacterial families important to the healthcare sector, as well as (ii) the presence of Salmonella in these coastal animals. The antimicrobial resistance pheno- and genotypes, as well as biocide susceptibility of Enterobacterales isolated from stranded marine mammals, were determined prior to their rehabilitation. All E. coli isolates (n = 27) were screened for virulence genes via DNA-based microarray, and twelve selected E. coli isolates were analyzed by whole-genome sequencing. Seventy-one percent of the Enterobacterales isolates exhibited a multidrug-resistant (MDR) pheno- and genotype. The gene bla_CMY (n = 51) was the predominant β-lactamase gene. In addition, bla_TEM-1 (n = 38), bla_SHV-33 (n = 8), bla_CTX-M-15 (n = 7), bla_OXA-1 (n = 7), bla_SHV-11 (n = 3), and bla_DHA-1 (n = 2) were detected. The most prevalent non-β-lactamase genes were sul2 (n = 38), strA (n = 34), strB (n = 34), and tet(A) (n = 34). Escherichia coli isolates belonging to the pandemic sequence types (STs) ST38, ST167, and ST648 were identified. Among Salmonella isolates (n = 18), S. Havana was the most prevalent serotype. The present study revealed a high prevalence of MDR bacteria and the presence of pandemic high-risk clones, both of which are indicators of anthropogenic antimicrobial pollution, in marine mammals.