Publikationsdatenbank

Does the in vitro egg hatch test predict the failure of benzimidazole treatment in Haemonchus contortus? (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Babják, Michal

Königová, Alžbeta

Urda Dolinská, Michaela

Kupčinskas, Tomas

Vadlejch, Jaroslav

von Samson-Himmelstjerna, Georg (WE 13)

Petkevičius, Saulius

Várady, Marián

Quelle

Parasite

Bandzählung: 28

Heftzählung: Special Issue

Seiten: Article Number 62

ISSN: 1776-1042

Sprache

Englisch

Verweise

URL (Volltext): https://www.parasite-journal.org/articles/parasite/full_html/2021/01/parasite210009/parasite210009.html

DOI: 10.1051/parasite/2021059

Pubmed: 34410223

Kontakt

Institut für Parasitologie und Tropenveterinärmedizin

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 62310
parasitologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Considerable research has been directed towards optimising in vitro tests that can diagnose resistance in pre-parasitic stages of parasites. The objective of this study was to compare the in vivo faecal egg count reduction test (FECRT), the in vitro egg hatch test (EHT), and the molecular determination of the frequency of a codon 200 allele of β-tubulin isotype 1 associated with benzimidazole resistance in larval stages of Haemonchus contortus obtained from infected goats. Animals were infected with composite infective doses representing 10, 20, 30, 40, 60, and 80% resistant alleles. Faecal samples for the EHT were collected on 28, 33, and 35 days post-infection. The results of the in vivo FECRT indicated that albendazole treatment reduced infections consisting of composite doses of 10, 20, 30, 40, 60, and 80% larvae of the resistant isolate by 91.3, 78.0, 63.3, 48.4, 36.5, and 41.4%, respectively. The drug concentration at which 50% of the eggs were prevented from developing hatching larvae (ED50) in the in vitro EHT varied from 0.09 ± 0.01 to 15.63 ± 12.10 μg/mL thiabendazole. The results of the in vitro EHT indicated that the test could estimate in vivo resistance well. The EHT could thus accurately estimate the in vivo efficacy of the drug and percentage of the resistance allele in the population using hatching parameters in delineation doses. This finding was also supported by comparing the FECRT data to the hatching percentages in the EHT on 30 goat farms in Slovakia with natural mixed infections of gastrointestinal parasites.