Publikationsdatenbank

Absence of porcine endogenous retrovirus (PERV) production from pig lymphoma cell lines (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Krüger, Luise

Böttger, Johanna

Huang, Christene A.

Denner, Joachim (WE 5)

Quelle

Virus research : an international journal of molecular and cellular virology

Bandzählung: 295

Seiten: Artikel 198286

ISSN: 0168-1702

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/abs/pii/S016817022031193X

DOI: 10.1016/j.virusres.2020.198286

Pubmed: 33418025

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Porcine endogenous retroviruses (PERVs) -A and -B are integrated in the genome of all pigs, whereas PERV-C is found in many, but not all pigs. Some immortalized pig cell lines, among them lymphoma cells, but also mitogen activated primary lymphocytes have been shown to release virus particles, which were able to infect human cells and some of them were recombinant PERV-A/C. Since retroviruses can induce lymphomas, two newly established pig lymphoma cell lines and an older one (L23) were analysed for PERV expression. All three lines harboured PERV-A, PERV-B and PERV-C proviruses, but PERV-A/C recombinants were found only in the genome of L23 cells. The expression at the RNA level was very low and no protein expression and particle release was observed, suggesting that PERVs were not involved in the pathogenesis of these lymphomas. However, all three cell lines were infected with the porcine lymphotropic herpesvirus-3 (PLHV-3), which may have been involved in lymphoma development.