Publikationsdatenbank

Overview of anaplasmosis in Arab countries in North Africa and the Middle East, and optimizing a commercial c-ELISA for camels (2021)

Art

Hochschulschrift

Autor

Parvizi, Omid (WE 10)

Quelle

Berlin: Mensch und Buch Verlag, 2021 — III, 78 Seiten

Sprache

Englisch

Verweise

URL (Volltext): https://refubium.fu-berlin.de/handle/fub188/30962

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Anaplasmosis is a tick-borne disease with a great economic importance for cattle farming that causes disorders of the hemolymphatic and immune system. It is distributed worldwide in tropical and sub-tropical countries. The economic impact of the disease is significant for animal welfare and public health. This work provides comprehensive information on anaplasmosis through a literature review of 19 Arab countries in the North Africa and the Middle East. Screening of cattle sera from Egypt was performed using commercial cELISA and real time PCR. Validation of a ‘bovine’ cELISA for use in camel sera, a ROC curve analysis was used to estimate the cutoff value, sensitivity and specificity. The number of anaplasmosis positive samples using molecular assays ranged from 4.4-61.7% in cattle and sheep in Algeria; 14.08-67.37% in cattle in Egypt; 7.5- 71% in dogs and small ruminants in Morocco; 0.6-69.6% in cattle and goats in Tunisia; 6.1-24.4% in cattle and dogs in Sudan; 62.6% in sheep in Iraq; 39.5% in dogs in Jordan; 1.6% in dogs in Qatar and 15.5-38.1% in small ruminants in Saudi Arabia. In Saudi Arabia, a high number of anaplasmosis positive samples from slaughter animals was noticed. In Egypt, cattle sera revealed a seroprevalence of 18.46% (CI: 15.8-21.4%) and 5.3% (CI: 3.8-7.1%) using cELISA or real-time PCR. Some of the sera (3.95%) were also positive for C. burnetii-specific antibodies. The best cutoff value of cELISA was calculated to be 0.42 (p <0.001) for camels sera whereas this value for cattle sera was 0.4022. Trace immanence of bovine ELISA νѕ. camel ELISA methods was shown as scatterplot. Prevalence in camels was analysed finally with a cutoff of 0.42. In New Valley Delta and Eastern Desert domain prevalences of 47.4% und 46.4% were found, respectively. The simulation for 2,300 generated data with 10% error allowed resulted in 97.8% specificity. The initial aims of the thesis i.e. to write a comprehensive review fo anaplasmosis for Northern Africa and the Near East, to adopt a ‚bovine‘ cELISA for use in camel sera and a preliminary study seroprevalence study for anaplasmosis in Egypt were succesfully fullfilled.