Publication Database

Biomimetic cell cultures:
techniques and terminology (2022)

Art

Poster

Autoren

Fisch, Anne-Sophie (WE 1)

Tinhofer-Keilholz, Ingeborg

Plendl, Johanna (WE 1)

Kongress

33rd Congress of the European Association of Veterinary Anatomists

Ghent, Belgium, 28. – 31.07.2021

Quelle

Anatomia, histologia, embryologia

Bandzählung: 51

Heftzählung: S1

Seiten: 21

ISSN: 0340-2096

Sprache

Englisch

Verweise

URL (Volltext): https://onlinelibrary.wiley.com/doi/10.1111/ahe.12759

DOI: 10.1111/ahe.12759

Kontakt

Institut für Veterinär-Anatomie

Koserstr. 20
14195 Berlin
+49 30 838 75784
anatomie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Introduction:
Cell culture techniques are in compliance with animal welfare and indispensable for many applications including drug de-velopment and personalized medicine.3D and 4D cultures recently replace traditional 2D in vitro models. Inconsistent terminology complicates key-word searches as well as choice and comparison of models. This study aims to present an overview of biomimetic three- dimensional approaches based on lit-erature search and own data.

Materials and Methods:
Literature search was carried out via elec-tronic databases like pubmed, google scholar and library portal Primo. Own studies include 3D cultures of endothelial or tumor cells for different research questions concerning angiogenesis and vascu-logenesis and tumor-derived spheroids and organoids.

Results:
3D cultures are grown on different scaffolds or matrices or in bioreactors. Co-cultures of two or more cell types such as skin constructs with endothelial cells emulate vascularization. Frequently used 3D biomimetic models are spheroids, organoids and embry-oids. Cultured on non-adherent surfaces, cells cluster to spherical structures by self-assembly, so-called spheroids.In contrast, organoids, embryoids and gastruloids are pluripotent embryonic stem cells, which organize upon incubation in specific growth factors and extracellular matrices. Tumoroids are based on cancer stem cells. Organs-on-chips consist of 3D polymeric micro-channels lined by cells exposed to biomechanical forces. 4D cell cul-tures include analysis of changes of cellular properties over time (4th dimension).

Conclusion:
Biomimetic cell cultures pave the way for new treat-ment options and a better understanding of cell biology in animals and humans. Latest therapeutic approaches of personalized medi-cine are based on those cultures from cancer patients.