Publikationsdatenbank

Proposal of epidemiological cutoff values for apramycin 15 μg and florfenicol 30 μg disks applicable to Staphylococcus aureus (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Costa, Sofia Santos

Ferreira, Carolina

Ribeiro, Rute

Feßler, Andrea T. (WE 7)

Schink, Anne-Kathrin (WE 7)

Kadlec, Kristina

Kaspar, Heike

Amaro, Ana

Albuquerque, Teresa

Abrantes, Patrícia

Morais, Catarina

Pomba, Constança

Schwarz, Stefan (WE 7)

Couto, Isabel

Quelle

Microbial drug resistance : MDR ; mechanisms, epidemiology, and disease

Bandzählung: 27

Heftzählung: 11

Seiten: 1555 – 1559

ISSN: 1076-6294

Sprache

Englisch

Verweise

URL (Volltext): https://www.liebertpub.com/doi/10.1089/mdr.2020.0402

DOI: 10.1089/mdr.2020.0402

Pubmed: 33956523

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Apramycin and florfenicol are two antimicrobial agents exclusively used in veterinary medicine. Resistance determinants to these antimicrobial agents have been described in several staphylococci, yet no inhibition zone-based epidemiological cutoff (ECOFF) values are available to detect populations harboring resistance mechanisms. In this study, we propose disk diffusion inhibition zone ECOFF values of Staphylococcus aureus for apramycin and florfenicol. The susceptibility to apramycin and florfenicol was evaluated by disk diffusion of five S. aureus collections, comprising 352 isolates of animal (n = 265) and human (n = 87) origin. The aggregated distributions of inhibition zone diameters were analyzed by the normalized resistance interpretation method to obtain normalized wild-type (WT) population distributions and corresponding ECOFF values. The putative WT populations of S. aureus were characterized by an inhibition zone ≥15 mm (ECOFF = 15 mm) for apramycin and ≥21 mm for florfenicol (ECOFF = 21 mm). Five nonwild-type (NWT) isolates were detected for apramycin, all without inhibition zone and harboring the apmA gene, whereas five NWT isolates were identified for florfenicol, all carrying the fexA gene. The proposed ECOFF values for apramycin and florfenicol may be a valuable tool in future antimicrobial resistance monitoring and surveillance studies to identify S. aureus NWT populations toward these antimicrobial agents.