Publikationsdatenbank

Molecular MR-imaging for noninvasive quantification of the anti-inflammatory effect of targeting interleukin-1β in a mouse model of aortic aneurysm (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Brangsch, Julia (WE 11)

Reimann, Carolin (WE 11)

Kaufmann, Jan Ole

Adams, Lisa Christine

Onthank, David

Thöne-Reineke, Christa (WE 11)

Robinson, Simon

Wilke, Marco

Weller, Michael

Buchholz, Rebecca

Karst, Uwe

Botnar, Rene

Hamm, Bernd

Makowski, Marcus Richard

Quelle

Molecular imaging : official journal of the Society for Molecular Imaging

Bandzählung: 19

Seiten: Artikel 1536012120961875

ISSN: 1536-0121

Sprache

Englisch

Verweise

URL (Volltext): https://journals.sagepub.com/doi/pdf/10.1177/1536012120961875

DOI: 10.1177/1536012120961875

Pubmed: 33216687

Kontakt

Institut für Tierschutz, Tierverhalten und Versuchstierkunde

Königsweg 67
14163 Berlin
+49 30 838 61146
tierschutz@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background:
Molecular-MRI is a promising imaging modality for the assessment of abdominal aortic aneurysms (AAAs). Interleukin-1β (IL-1β) represents a new therapeutic tool for AAA-treatment, since pro-inflammatory cytokines are key-mediators of inflammation. This study investigates the potential of molecular-MRI to evaluate therapeutic effects of an anti-IL-1β-therapy on AAA-formation in a mouse-model.

Methods:
Osmotic-minipumps were implanted in apolipoprotein-deficient-mice (N = 27). One group (Ang-II+01BSUR group, n = 9) was infused with angiotensin-II (Ang-II) for 4 weeks and received an anti-murine IL-1β-antibody (01BSUR) 3 times. One group (Ang-II-group, n = 9) was infused with Ang-II for 4 weeks but received no treatment. Control-group (n = 9) was infused with saline and received no treatment. MR-imaging was performed using an elastin-specific gadolinium-based-probe (0.2 mmol/kg).

Results:
Mice of the Ang-II+01BSUR-group showed a lower aortic-diameter compared to mice of the Ang-II-group and control mice (p < 0.05). Using the elastin-specific-probe, a significant decrease in elastin-destruction was observed in mice of the Ang-II+01BSUR-group. In vivo MR-measurements correlated well with histopathology (y = 0.34x-13.81, R2 = 0.84, p < 0.05), ICP-MS (y = 0.02x+2.39; R2 = 0.81, p < 0.05) and LA-ICP-MS. Immunofluorescence and western-blotting confirmed a reduced IL-1β-expression.

Conclusions:
Molecular-MRI enables the early visualization and quantification of the anti-inflammatory-effects of an IL-1β-inhibitor in a mouse-model of AAAs. Responders and non-responders could be identified early after the initiation of the therapy using molecular-MRI.