Publikationsdatenbank

Functional scaffold-free bone equivalents induce osteogenic and angiogenic processes in a human in vitro fracture hematoma model (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Pfeiffenberger, Moritz (WE 11)

Damerau, Alexandra

Ponomarev, Igor

Bucher, Christian H.

Chen, Yuling

Barnewitz, Dirk

Thöne-Reineke, Christa (WE 11)

Hoff, Paula

Buttgereit, Frank

Gaber, Timo

Lang, Annemarie

Quelle

Journal of bone and mineral research : JBMR ; the official journal of the American Society for Bone and Mineral Research ASBMR

Bandzählung: 36

Heftzählung: 6

Seiten: 1189 – 1201

ISSN: 0884-0431

Sprache

Englisch

Verweise

URL (Volltext): https://asbmr.onlinelibrary.wiley.com/doi/10.1002/jbmr.4267

DOI: 10.1002/jbmr.4267

Pubmed: 33534144

Kontakt

Institut für Tierschutz, Tierverhalten und Versuchstierkunde

Königsweg 67
14163 Berlin
+49 30 838 61146
tierschutz@vetmed.fu-berlin.de

Abstract / Zusammenfassung

After trauma, the formed fracture hematoma within the fracture gap contains all the important components (immune/stem cells, mediators) to initiate bone regeneration immediately. Thus, it is of great importance but also the most susceptible to negative influences. To study the interaction between bone and immune cells within the fracture gap, up-to-date in vitro systems should be capable to recapitulate cellular and humoral interactions and the physicochemical microenvironment (e.g. hypoxia). Here, we first developed and characterized scaffold-free bone-like constructs (SFBCs) which were produced from bone marrow-derived mesenchymal stromal cells (MSCs) using a macroscale mesenchymal condensation approach. SFBCs revealed permeating mineralization characterized by increased bone volume (μCT, histology) and expression of osteogenic markers (RUNX2, SPP1, RANKL). Fracture hematoma (FH) models, consisting of human peripheral blood (immune cells) mixed with MSCs, were co-cultivated with SFBCs under hypoxic conditions. As a result, FH models revealed an increased expression of osteogenic (RUNX2, SPP1), angiogenic (MMP2, VEGF), HIF-related (LDHA, PGK1) and inflammatory (IL6, IL8) markers after 12 and 48h co-cultivation. Osteogenic and angiogenic gene expression of the FH indicate the osteoinductive potential and, thus, the biological functionality of the SFBCs. IL-6, IL-8, GM-CSF, and MIP-1β were detectable within the supernatant after 24 and 48h of co-cultivation. To confirm the responsiveness of our model to modifying substances (e.g. therapeutics), we used deferoxamine (DFO) well-known to induce a cellular hypoxic adaptation response. Indeed, DFO particularly increased hypoxia-adaptive, osteogenic and angiogenic processes within the FH models but had little effect on the SFBCs indicating different response dynamics within the co-cultivation system. Therefore, based on our data we have successfully modeled processes within the initial fracture healing phase in vitro and concluded that the crosstalk between bone and immune cells in the initial fracture healing phase is of particular importance for preclinical studies.