Publikationsdatenbank

Blood-brain barrier protein claudin-5 expressed in paired Xenopus laevis oocytes mediates cell-cell interaction (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Brunner, Nora (WE 2)

Stein, Laura (WE 2)

Cornelius, Valeria (WE 2)

Knittel, Ria

Fallier-Becker, Petra

Amasheh, Salah (WE 2)

Quelle

Frontiers in physiology

Bandzählung: 11

Seiten: Article 857

ISSN: 1664-042x

Sprache

Englisch

Verweise

URL (Volltext): https://www.frontiersin.org/articles/10.3389/fphys.2020.00857/full

DOI: 10.3389/fphys.2020.00857

Pubmed: 32848831

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Claudin-5 determines the sealing properties of blood-brain barrier tight junctions and its function is impaired in neurodegenerative and neuroinflammatory disorders. Focusing on the contribution of claudin-5 to the trans-interaction within the tight junction seal, we used Xenopus laevis oocytes as an expression system. Cells were clustered and challenged in a novel approach for the analysis of claudin interaction. We evaluated the strengthening effect of claudin-5 to cell-cell-connection in comparison to claudin-3. Application of a hydrostatic pressure impulse on clustered control oocyte pairs revealed a reduction of contact areas. In contrast, combinations with both oocytes expressing claudins maintained an enhanced connection between the cells (cldn5-cldn5, cldn3-cldn3). Strength of interaction was increased by both claudin-3 and claudin-5. This novel approach allowed an analysis of single claudins contributing to tight junction integrity, characterizing homophilic and hetrophilic trans-interaction of claudins. To test a new screening approach for barrier effectors, exemplarily, this 2-cell model of oocytes was used to analyze the effect of the absorption enhancer sodium caprate on the oocyte pairs.