Publikationsdatenbank

Characterization of porcine LA-MRSA CC398 and CC9 isolates from Germany and China via Biolog Phenotype MicroArray™ (2020)

Art

Poster

Autoren

Krüger, Henrike (WE 7)

Ji, Xing

Feßler, Andrea T. (WE 7)

Jiang, Nansong

Bartel, Alexander (WE 16)

Tedin, Karsten (WE 7)

Maurischat, Sven

Wang, Yang

Wu, Congming

Schwarz, Stefan (WE 7)

Kongress

Zoonoses 2020 - International Symposium on Zoonoses Research

online, 15. – 16.10.2020

Quelle

Sprache

Englisch

Verweise

URL (Volltext): https://evis.events/event/119/contributions/2473/contribution.pdf

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Objective:
In this study, we investigated porcine livestock-associated methicillin-resistant Staphylococcus aureus (LAMRSA) of clonal complex (CC) 398 and 9 for their metabolic properties in order to explain their different prevalence in Germany and China.

Methodology:
Overall, 17 porcine LA-MRSA isolates of CC398 (n = 7) and CC9 (n = 10) collected in Germany (n = 10) and China (n = 7) were investigated. At least two Biolog Phenotype MicroArray™ runs with 96-well plates containing carbon sources, osmolytes and different pH conditions were performed. Metabolic substrate utilization was quantified via the Area Under the Curve (AUC) of the respiration curves. Sparse partial least squares discriminant analysis (sPLS-DA) was applied to determine the 50 most informative out of the 379 substances tested in order to distinguish the isolates based on CC and origin.

Results:
The selected substrates included sugars, sodium salts and amino acids among others. Using sPLS-DA, it was possible to cluster the isolates in four groups depending on CC and origin. The Chinese MRSA-CC9 showed some major variations in the metabolism of the carbon sources D-malic acid, m-tartaric acid and sedoheptulosan. Lower AUC values point towards a reduced usage in relation to the other groups.

Conclusion:
The porcine LA-MRSA isolates from Germany and China displayed in part different metabolic properties. However, further analyses are needed to detect the reasons for the varying epidemiological success of the two CC.