Publikationsdatenbank

Provisional establishment of quality control strains for antimicrobial susceptibility testing of Mycoplasma hyorhinis (2020)

Art

Poster

Autoren

Käbisch, Lisa (WE 6)

Schink, Anne-Kathrin (WE 7)

Schwarz, Stefan (WE 7)

Kongress

Zoonoses 2020 - International Symposium on Zoonoses Research

online, 15. – 16.10.2020

Quelle

Sprache

Englisch

Verweise

URL (Volltext): https://evis.events/event/119/contributions/2472/contribution.pdf

Kontakt

Institut für Immunologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51834
immunologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background and objectives:
Quality control (QC) strains are important to ensure the functionality of a test system. The Clinical and Laboratory Standards Institute (CLSI) has approved several QC strains for antimicrobial susceptibility testing (AST) of numerous bacteria, but not yet for Mycoplasma hyorhinis. The aim of this study was to investigate CLSI-approved QC strains for their suitability in AST of M. hyorhinis.

Material and methods:
Two QC strains were tested using commercial microtiter plates that contain 18 antimicrobial agents and Mycoplasma-specific Friis medium. Enterococcus faecalis ATCC® 29212 and Staphylococcus aureus ATCC® 29213 were incubated for 24 h, 48 h and 72 h at 35 ℃ ± 2 ℃ in ambient air. The prolonged incubation time relates to the incubation time suitable for M. hyorhinis. The strains were tested at least 11 times at independent occasions.

Results:
In the Friis medium, the MICs of the following strain-antimicrobial agent combinations were mainly within the acceptable ranges: E. faecalis ATCC® 29212 (gentamicin, streptomycin, enrofloxacin, florfenicol, erythromycin, tilmicosin, tulathromycin, tylosin, doxycycline) and S. aureus ATCC® 29213 (neomycin,
ciprofloxacin, marbofloxacin, clindamycin, pirlimycin, linezolid, tiamulin, quinupristin-dalfopristin, tetracycline).

Conclusion:
As long as no M. hyorhinis QC strain is approved, we propose the provisional use of the two ATCC® strains 29212 and 29213 as QC strains for M. hyorhinis AST in Friis medium.