Publication Database

Identification of a bla_VIM-1-Carrying IncA/C2 multiresistance plasmid in an Escherichia coli isolate recovered from the German food chain (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Pauly, Natalie

Hammerl, Jens Andre

Grobbel, Mirjam

Käsbohrer, Annemarie

Tenhagen, Bernd-Alois

Malorny, Burkhard

Schwarz, Stefan (WE 7)

Meemken, Diana (WE 8)

Irrgang, Alexandra

Quelle

Microorganisms : open access journal

Bandzählung: 9

Heftzählung: 1

Seiten: Artikel 29

ISSN: 2076-2607

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/2076-2607/9/1/29

DOI: 10.3390/microorganisms9010029

Pubmed: 33374123

Kontakt

Institut für Lebensmittelsicherheit und -hygiene

Königsweg 69
14163 Berlin
+49 30 838 62551 / 52790
lebensmittelhygiene@vetmed.fu-berlin.de / fleischhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Within the German national monitoring of zoonotic agents, antimicrobial resistance determination also targets carbapenemase-producing (CP) Escherichia coli by selective isolation from food and livestock. In this monitoring in 2019, the CP E. coli 19-AB01133 was recovered from pork shoulder. The isolate was assigned to the phylogenetic group B1 and exhibited the multi-locus sequence-type ST5869. Molecular investigations, including whole genome sequencing, of 19-AB01133 revealed that the isolate carried the resistance genes bla_VIM-1, bla_SHV-5 and bla_CMY-13 on a self-transmissible IncA/C2 plasmid. The plasmid was closely related to the previously described VIM-1-encoding plasmid S15FP06257_p from E. coli of pork origin in Belgium. Our results indicate an occasional spread of the bla_VIM-1 gene in Enterobacteriaceae of the European pig population. Moreover, the blaVIM-1 located on an IncA/C2 plasmid supports the presumption of a new, probably human source of carbapenemase-producing Enterobacteriaceae (CPE) entering the livestock and food chain sector.