Publikationsdatenbank

ChromID® CARBA agar fails to detect Carbapenem-resistant Enterobacteriaceae with slightly reduced susceptibility to Carbapenems (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Pauly, Natalie

Hammerl, Jens A.

Grobbel, Mirjam

Tenhagen, Bernd-Alois

Käsbohrer, Annemarie

Bisenius, Sandra

Fuchs, Jannika

Horlacher, Sabine

Lingstädt, Holger

Mauermann, Ute

Mitro, Silke

Müller, Margit

Rohrmann, Stefan

Schiffmann, Arthur P.

Stührenberg, Birgit

Zimmermann, Pia

Schwarz, Stefan (WE 7)

Meemken, Diana (WE 8)

Irrgang, Alexandra

Quelle

Frontiers in microbiology

Bandzählung: 11

Seiten: Article 1678

ISSN: 1664-302x

Sprache

Englisch

Verweise

URL (Volltext): https://www.frontiersin.org/articles/10.3389/fmicb.2020.01678/full

DOI: 10.3389/fmicb.2020.01678

Pubmed: 32849351

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

After first detections of carbapenemase-producing Enterobacteriaceae (CPE) in animals, the European Union Reference Laboratory for Antimicrobial Resistance has provided a protocol for the isolation of carbapenemase-producing Escherichia (E.) coli from cecum content and meat. Up to now, only few isolates were recovered using this procedure. In our experience, the choice of the selective agar is important for the efficacy of the method. Currently, the use of the prevailing method fails to detect CPE that exhibit a low resistance against carbapenems. Thus, this study aims to evaluate the suitability of selective media with antibiotic supplements and commercial ChromID® CARBA agar for a reliable CPE detection. For comparative investigations, detection of freeze-dried carbapenemase-resistant bacteria was studied on different batches of the ChromID® CARBA agar as well as on MacConkey agar supplemented with 1 mg/L cefotaxime and 0.125 mg/L meropenem (McC+CTX+MEM). The suitability of the different media was assessed within a time of 25 weeks, starting at least six weeks before expiration of the media. Carbapenem-resistant isolates exhibiting a serine-based hydrolytic resistance mechanism (e.g., bla_KPC genes) were consistently detected over 25 weeks on the different media. In contrast, carbapenemase producers with only slightly reduced susceptibility and exhibiting a zinc-catalyzed activity (e.g., bla_VIM, bla_NDM, and bla_IMP) could only be cultivated on long-time expired ChromID® CARBA, but within the whole test period on McC+CTX+MEM. Thus, ChromID® CARBA agar appears to be not suitable for the detection of CPE with slightly increased minimum inhibitory concentrations (MIC) against carbapenems, which have been detected in German livestock and thus, are of main interest in the national monitoring programs. Our data are in concordance with the results of eleven state laboratories that had participated in this study with their ChromID® CARBA batches routinely used for the German CPE monitoring. Based on the determined CPE detection rate, we recommend the use of McC+CTX+MEM for monitoring purposes. This study indicates that the use of ChromID® CARBA agar might lead to an underestimation of the current CPE occurrence in food and livestock samples.