Publikationsdatenbank

The cell-wall structure variation in Mycobacterium tuberculosis with different drug sensitivity using Raman spectroscopy in the high-wavenumber region (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Zyubin, Andrey

Lavrova, Anastasia

Manicheva, Olga

Dogonadze, Marine

Belik, Vitaly (WE 16)

Demin, Maxim

Samusev, Ilia

Quelle

Laser Physics Letters

Bandzählung: 17

Heftzählung: 6

Seiten: Artikel 065602

ISSN: 1612-2011

Sprache

Englisch

Verweise

URL (Volltext): https://iopscience.iop.org/article/10.1088/1612-202X/ab8796

DOI: 10.1088/1612-202X/ab8796

Kontakt

Institut für Veterinär-Epidemiologie und Biometrie

Königsweg 67
14163 Berlin
+49 30 838 56034
epi@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Over the past decade Raman spectroscopy has been successfully used to study the cell structure of various pathogens, including M. tuberculosis (MbT). It is especially important due to the high ability of pathogen to become resistant to anti-tuberculosis drugs usually leading to a lethal outcome. In the present study we investigate deactivated MbT strains with different drug sensitivity (extra-, multi- and sensitive drug resistance) of the Beijing family. Samples have been obtained from patients suffering from pulmonary and extra-pulmonary forms of tuberculosis. Samples have been investigated by Raman spectrometry using a diode-pumped solid-state argon (514 nm) laser excitation source in the high-wavenumber region. As a result, protein and lipid vibrational bands for strain discrimination with various drug resistances have been determined. The obtained results can be used as fast diagnostic tool development for screening MbT strains in clinical medical practice.