Publikationsdatenbank

Dietary Mg²⁺ intake and the Na⁺/Mg²⁺ exchanger SLC41A1 influence components of mitochondrial energetics in murine cardiomyocytes (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Tatarkova, Zuzana

de Baaij, Jeroen H. F.

Grendar, Marian

Aschenbach, Jörg R. (WE 2)

Racay, Peter

Bos, Caro

Sponder, Gerhard (WE 2)

Hoenderop, Joost G. J.

Röntgen, Monika

Turcanova Koprusakova, Monika

Kolisek, Martin

Quelle

International journal of molecular sciences

Bandzählung: 21

Heftzählung: 21

Seiten: Artikel 8221

ISSN: 1422-0067

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/1422-0067/21/21/8221

DOI: 10.3390/ijms21218221

Pubmed: 33153064

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Cardiomyocytes are among the most energy-intensive cell types. Interplay between the components of cellular magnesium (Mg) homeostasis and energy metabolism in cardiomyocytes is poorly understood. We have investigated the effects of dietary Mg content and presence/functionality of the Na+/Mg2+ exchanger SLC41A1 on enzymatic functions of selected constituents of the Krebs cycle and complexes of the electron transport chain (ETC). The activities of aconitate hydratase (ACON), isocitrate dehydrogenase (ICDH), α-ketoglutarate dehydrogenase (KGDH), and ETC complexes CI-CV have been determined in vitro in mitochondria isolated from hearts of wild-type (WT) and Slc41a1-/- mice fed a diet with either normal or low Mg content. Our data demonstrate that both, the type of Mg diet and the Slc41a1 genotype largely impact on the activities of enzymes of the Krebs cycle and ETC. Moreover, a compensatory effect of Slc41a1-/- genotype on the effect of low Mg diet on activities of the tested Krebs cycle enzymes has been identified. A machine-learning analysis identified activities of ICDH, CI, CIV, and CV as common predictors of the type of Mg diet and of CII as suitable predictor of Slc41a1 genotype. Thus, our data delineate the effect of dietary Mg content and of SLC41A1 functionality on the energy-production in cardiac mitochondria.