Publikationsdatenbank

Left-handed DNA-PAINT for improved super-resolution imaging in the nucleus (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Geertsema, H. J.

Aimola, G. (WE 5)

Fabricius, V.

Fuerste, J. P.

Kaufer, B. B. (WE 5)

Ewers, H.

Quelle

Nature biotechnology : the science and business of biotechnology

Bandzählung: 39

Heftzählung: 5

Seiten: 551 – 554

ISSN: 1087-0156

Sprache

Englisch

Verweise

URL (Volltext): https://www.nature.com/articles/s41587-020-00753-y

DOI: 10.1038/s41587-020-00753-y

Pubmed: 33398151

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

DNA point accumulation in nanoscale topography (DNA-PAINT) increases the resolution and multiplexing capabilities of super-resolution imaging, but cellular DNA interferes with DNA-DNA hybridization between target and probe in the nucleus. Here, we introduce left-handed DNA (L-DNA) oligomers that do not hybridize to natural right-handed DNA (R-DNA) and demonstrate that L-DNA-PAINT has the same specificity and multiplexing capability as R-DNA-PAINT, but improves the imaging of nuclear targets by substantially reducing background signal.