Publication Database

Effects of selected insecticidal substances on mRNA transcriptome in larvae of Apis mellifera (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Kablau, Arne (WE 3)

Eckert, Jakob H.

Pistorius, Jens

Sharbati, Soroush (WE 3)

Einspanier, Ralf (WE 3)

Quelle

Pesticide biochemistry and physiology

Bandzählung: 170

Seiten: Artikel 104703

ISSN: 1095-9939

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/abs/pii/S004835752030198X?via%3Dihub

DOI: 10.1016/j.pestbp.2020.104703

Pubmed: 32980071

Kontakt

Institut für Veterinär-Biochemie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62225
biochemie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

For the last decade, scientists have reported a loss of honeybee colonies. Multiple factors like parasites, pathogens and pesticides are dealt as possible drivers of honeybee losses. In particular, insecticides are considered as a major factor of pollinator poisoning. We applied sublethal concentrations of four insecticidal substances to honeybee larval food and analyzed the effects on transcriptome. The aim was to identify candidate genes indicating early negative impacts after application of insecticidal substances. Honeybee larvae were kept in-vitro under hive conditions (34-35 °C) and fed with dimethoate, fenoxycarb, chlorantraniliprole and flupyradifurone in sublethal concentrations between day 3-6 after grafting. Larvae at day 4, 6 and 8 were sampled and their transcriptome analyzed. By use of a RT-qPCR array differences in gene expression of selected gene families (immune system, development detoxification) were measured. Targets mainly involved in development, energy metabolism and the immune system were significantly affected by the insecticidal substances tested, selectively inducing genes of the detoxification system, immune response and nutritional stress.