Publication Database

B cell speed and B-FDC contacts in germinal centers determine plasma cell output via swiprosin-1/EFhd2 (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Reimer, Dorothea

Meyer-Hermann, Michael

Rakhymzhan, Asylkhan

Steinmetz, Tobit

Tripal, Philipp

Thomas, Jana

Boettcher, Martin

Mougiakakos, Dimitrios

Schulz, Sebastian R.

Urbanczyk, Sophia

Hauser, Anja E.

Niesner, Raluca A. (WE 2)

Mielenz, Dirk

Quelle

Cell reports

Bandzählung: 32

Heftzählung: 6

Seiten: Artikel 108030

ISSN: 2211-1247

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/pii/S2211124720310159

DOI: 10.1016/j.celrep.2020.108030

Pubmed: 32783949

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Plasma cells secreting affinity-matured antibodies develop in germinal centers (GCs), where B cells migrate persistently and directionally over defined periods of time. How modes of GC B cell migration influence plasma cell development remained unclear. Through genetic deletion of the F-actin bundling protein Swiprosin-1/EF-hand domain family member 2 (EFhd2) and by two-photon microscopy, we show that EFhd2 restrains B cell speed in GCs and hapten-specific plasma cell output. Modeling the GC reaction reveals that increasing GC B cell speed promotes plasma cell generation. Lack of EFhd2 also reduces contacts of GC B cells with follicular dendritic cells in vivo. Computational modeling uncovers that both GC output and antibody affinity depend quantitatively on contacts of GC B cells with follicular dendritic cells when B cells migrate more persistently. Collectively, our data explain how GC B cells integrate speed and persistence of cell migration with B cell receptor affinity.