Publication Database

The amyloid-beta rich CNS environment alters myeloid cell functionality independent of their origin (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Drost, Natalia

Houtman, Judith

Cseresnyés, Zoltán

Niesner, Raluca (WE 2)

Rinnenthal, Jan-Leo

Miller, Kelly R.

Prokop, Stefan

Heppner, Frank L.

Quelle

Scientific reports

Bandzählung: 10

Heftzählung: 1

Seiten: Artikel 7152

ISSN: 2045-2322

Sprache

Englisch

Verweise

URL (Volltext): http://www.nature.com/articles/s41598-020-63989-3

DOI: 10.1038/s41598-020-63989-3

Pubmed: 32346002

Kontakt

Institut für Veterinär-Physiologie

Oertzenweg 19 b
14163 Berlin
+49 30 838 62600
physiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Microglia, the innate immune cells of the central nervous system (CNS) survey their surroundings with their cytoplasmic processes, phagocytose debris and rapidly respond to injury. These functions are affected by the presence of beta-Amyloid (Aβ) deposits, hallmark lesions of Alzheimer's disease (AD). We recently demonstrated that exchanging functionally altered endogenous microglia with peripheral myeloid cells did not change Aβ-burden in a mouse model mimicking aspects of AD at baseline, and only mildly reduced Aβ plaques upon stimulation. To better characterize these different myeloid cell populations, we used long-term in vivo 2-photon microscopy to compare morphology and basic functional parameters of brain populating peripherally-derived myeloid cells and endogenous microglia. While peripherally-derived myeloid cells exhibited increased process movement in the non-diseased brain, the Aβ rich environment in an AD-like mouse model, which induced an alteration of surveillance functions in endogenous microglia, also restricted functional characteristics and response to CNS injury of newly recruited peripherally-derived myeloid cells. Our data demonstrate that the Aβ rich brain environment alters the functional characteristics of endogenous microglia as well as newly recruited peripheral myeloid cells, which has implications for the role of myeloid cells in disease and the utilization of these cells in Alzheimer's disease therapy.