Publication Database

Immunocompatibility and non-thrombogenicity of gelatin-based hydrogels (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Krüger-Genge, A.

Tondera, C.

Hauser, S.

Braune, S.

Görs, J.

Roch, T.

Klopfleisch, R. (WE 12)

Neffe, A. T.

Lendlein, A.

Pietzsch, J.

Jung, F.

Quelle

Clinical hemorheology and microcirculation : blood flow and vessels

Bandzählung: 77

Heftzählung: 3

Seiten: 335 – 350

ISSN: 1386-0291

Sprache

Englisch

Verweise

URL (Volltext): https://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch201028

DOI: 10.3233/CH-201028

Pubmed: 33337355

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Immunocompatibility and non-thrombogenicity are important requirements for biomedical applications such as vascular grafts. Here, gelatin-based hydrogels formed by reaction of porcine gelatin with increasing amounts of lysine diisocyanate ethyl ester were investigated in vitro in this regard. In addition, potential adverse effects of the hydrogels were determined using the "Hen's egg test on chorioallantoic membrane" (HET-CAM) test and a mouse model.The study revealed that the hydrogels were immunocompatible, since complement activation was absent and a substantial induction of reactive oxygen species generating monocytes and neutrophils could not be observed in whole human blood. The density as well as the activation state of adherent thrombocytes was comparable to medical grade polydimethylsiloxane, which was used as reference material. The HET-CAM test confirmed the compatibility of the hydrogels with vessel functionality since no bleedings, thrombotic events, or vessel destructions were observed. Only for the samples synthesized with the highest LDI amount the number of growing blood vessels in the CAM was comparable to controls and significantly higher than for the softer materials. Implantation into mice showed the absence of adverse or toxic effects in spleen, liver, or kidney, and only a mild lymphocytic activation in the form of a follicular hyperplasia in draining lymph nodes (slightly increased after the implantation of the material prepared with the lowest LDI content). These results imply that candidate materials prepared with mid to high amounts of LDI are suitable for the coating of the blood contacting surface of cardiovascular implants.