Publikationsdatenbank

Novel protocol for the in vitro cultivation and Magnetic-activated cell sorting (MACS) of highly purified neonatal murine microglia and astrocytes (2020)

Art

Poster

Autoren

Zelenka, Laura (WE 7)

Pägelow, Dennis (WE 7)

Krüger, Christina

Seele, Jana

van Vorst, Kira (WE 7)

Ebner, Frederike (WE 6)

Rausch, Sebastian (WE 6)

Hartmann, Susanne (WE 6)

Lehnardt, Seija

Fulde, Marcus (WE 7)

Kongress

Zoonoses 2020 - International Symposium on Zoonoses Research

online, 15. – 16.10.2020

Quelle

Zoonoses 2020 – International Symposium on Zoonoses Research : online meeting; joint meeting of the German Research Platform for Zoonoses and the Research Network of Zoonotic Diseases : October 15th - 16th, 2020 : program and abstracts — [National Research Platform for Zoonoses and the Research Network of Zoonotic Infectious Diseases] (Hrsg.)

Berlin, 2020 — S. Beitrag ID:317

Sprache

Englisch

Verweise

URL (Volltext): https://evis.events/event/119/contributions/2454/contribution.pdf

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Konferenzband: https://www.openagrar.de/receive/openagrar_mods_00065483
Abstract: https://evis.events/event/119/contributions/2454/contribution.pdf

Infectious diseases of the central nervous system (CNS) are of major health concern with high mortality rates and frequent occurrence of convalescence, occasionally also including long-term sequelae. The crosstalk and reactivity of the cell type glia, especially microglia and astrocytes, have progressively gathered research attention in understanding proper brain function and defense to viral as well as bacterial infections. In this study, we modified previously established techniques by applying an optimized Magnetic-activated cell sorting (MACS) protocol using CD11b and ACSA-2 labeled MicroBeads for the isolation of microglia and astrocytes from neonatal mouse brains. Thereby, we achieved highly viable and pure (~99 %) microglia and astrocytes as confirmed by FACS analysis. By using this developed method, highly purified primary microglia and astrocytes, potentially with different genetic backgrounds, can be cultivated in vitro. This will facilitate the analysis of the cell-type specific involvement in the frame of CNS diseases by applying this highly useful quick and easy tool in future approaches.