Publication Database

Multi-species ELISA for the detection of antibodies against SARS-CoV-2 in animals (2021)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Wernike, Kerstin

Aebischer, Andrea

Michelitsch, Anna

Hoffmann, Donata

Freuling, Conrad

Balkema-Buschmann, Anne

Graaf, Annika

Müller, Thomas

Osterrieder, Nikolaus (WE 5)

Rissmann, Melanie

Rubbenstroth, Dennis

Schön, Jacob

Schulz, Claudia

Trimpert, Jakob (WE 5)

Ulrich, Lorenz

Volz, Asisa

Mettenleiter, Thomas

Beer, Martin

Quelle

Transboundary and emerging diseases

Bandzählung: 68

Heftzählung: 4

Seiten: 1779 – 1785

ISSN: 1865-1674

Sprache

Englisch

Verweise

URL (Volltext): https://onlinelibrary.wiley.com/doi/10.1111/tbed.13926

DOI: 10.1111/tbed.13926

Pubmed: 33191578

Kontakt

Institut für Virologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51833
virologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a pandemic with millions of infected humans and hundreds of thousands of fatalities. As the novel disease - referred to as COVID-19 - unfolded, occasional anthropozoonotic infections of animals by owners or caretakers were reported in dogs, felid species and farmed mink. Further species were shown to be susceptible under experimental conditions. The extent of natural infections of animals, however, is still largely unknown. Serological methods will be useful tools for tracing SARS-CoV-2 infections in animals once test systems are evaluated for use in different species. Here, we developed an indirect multi-species ELISA based on the receptor-binding domain (RBD) of SARS-CoV-2. The newly established ELISA was evaluated using 59 sera of infected or vaccinated animals, including ferrets, raccoon dogs, hamsters, rabbits, chickens, cattle and a cat, and a total of 220 antibody-negative sera of the same animal species. Overall, a diagnostic specificity of 100.0% and sensitivity of 98.31% were achieved, and the functionality with every species included in this study could be demonstrated. Hence, a versatile and reliable ELISA protocol was established that enables high-throughput antibody detection in a broad range of animal species, which may be used for outbreak investigations, to assess the seroprevalence in susceptible species or to screen for reservoir or intermediate hosts.