Publikationsdatenbank

Impact of intercellular crosstalk between epidermal keratinocytes and dermal fibroblasts on skin homeostasis (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Jevtić, Marijana

Löwa, Anna

Nováčková, Anna

Kováčik, Andrej

Kaessmeyer, Sabine (WE 1)

Erdmann, Gerrit

Vávrová, Kateřina

Hedtrich, Sarah

Quelle

Biochimica et biophysica acta : BBA. Molecular cell research

Bandzählung: 1867

Heftzählung: 8

Seiten: 118722

ISSN: 1879-2596

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/abs/pii/S016748892030080X

DOI: 10.1016/j.bbamcr.2020.118722

Pubmed: 32302667

Kontakt

Institut für Veterinär-Anatomie

Koserstr. 20
14195 Berlin
+49 30 838 75784
anatomie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Dermal fibroblasts seem critical for epidermal maturation and differentiation and recent work demonstrated that diseased fibroblasts may drive pathophysiological processes. Nevertheless, still very little is known about the actual crosstalk between epidermal keratinocytes and dermal fibroblasts and the impact of dermal fibroblasts on epidermal maturation and differentiation. Aiming for a more fundamental understanding of the impact of the cellular crosstalk between keratinocytes and fibroblasts on the skin homeostasis, we generated full-thickness skin equivalents with and without fibroblasts and subsequently analysed them for the expression of skin differentiation markers, their barrier function, skin lipid content and epidermal cell signalling. Skin equivalents without fibroblasts consistently showed an impaired differentiation and dysregulated expression of skin barrier and tight junction proteins, increased skin permeability, and a decreased skin lipid/protein ratio. Most interestingly, impaired Ras/Raf/ERK/MEK signalling was evident in skin equivalents without fibroblasts. Our data clearly indicate that the epidermal-dermal crosstalk between keratinocytes and fibroblasts is critical for adequate skin differentiation and that fibroblasts orchestrate epidermal differentiation processes.