Publikationsdatenbank

Estimation of total collagen volume:
a T1 mapping versus histological comparison study in healthy landrace pigs (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Faragli, A.

Merz, S. (WE 12)

Muzio, F. P. Lo

Doeblin, P.

Tanacli, R.

Kolp, C.

Abawi, D.

Ötvös, J.

Stehning, C.

Schnackenburg, B.

Pieske, B.

Post, H.

Klopfleisch, R. (WE 12)

Alogna, A.

Kelle, S.

Quelle

The international journal of cardiovascular imaging : X-Ray imaging, echocardiography, nuclear cardiology and magnetic resonance

Bandzählung: 36

Heftzählung: 9

Seiten: 1761 – 1769

ISSN: 1573-0743

Sprache

Englisch

Verweise

URL (Volltext): https://link.springer.com/article/10.1007%2Fs10554-020-01881-x

DOI: 10.1007/s10554-020-01881-x

Pubmed: 32409978

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Right ventricular biopsy represents the gold standard for the assessment of myocardial fibrosis and collagen content. This invasive technique, however, is accompanied by perioperative complications and poor reproducibility. Extracellular volume (ECV) measured through cardiovascular magnetic resonance (CMR) has emerged as a valid surrogate method to assess fibrosis non-invasively. Nonetheless, ECV provides an overestimation of collagen concentration since it also considers interstitial space. Our study aims to investigate the feasibility of estimating total collagen volume (TCV) through CMR by comparing it with the TCV measured at histology. Seven healthy Landrace pigs were acutely instrumented closed-chest and transported to the MRI facility for measurements. For each protocol, CMR imaging at 3T was acquired. MEDIS software was used to analyze T1 mapping and ECV for both the left ventricular myocardium (LVmyo) and left ventricular septum (LVseptum). ECV was then used to estimate TCVCMR at LVmyo and LVseptum following previously published formulas. Tissues were prepared following an established protocol and stained with picrosirius red to analyze the TCVhisto in LVmyo and LVseptum. TCV measured at LVmyo and LVseptum with both histology (8 ± 5 ml and 7 ± 3 ml, respectively) and T1-Mapping (9 ± 5 ml and 8 ± 6 ml, respectively) did not show any regional differences. TCVhisto and TCVCMR showed a good level of data agreement by Bland-Altman analysis. Estimation of TCV through CMR may be a promising way to non-invasively assess myocardial collagen content and may be useful to track disease progression or treatment response.