Publication Database

Preservation and processing of intestinal tissue for the assessment of histopathology (2021)

Art

Buchbeitrag

Autoren

Rieger, Juliane

Pelckmann, Lisa-Marie (WE 1)

Drewes, Barbara (WE 1)

Quelle

Animal models of allergic disease : methods and protocols — Kumi Nagamoto-Combs (Hrsg.)

1st edition 2021 Auflage

New York, NY: Humana Press, 2021. Methods in molecular biology ; 2223 — S. 267–280

ISBN: 978-1-0716-1001-5

Sprache

Englisch

Verweise

URL (Volltext): https://link.springer.com/protocol/10.1007/978-1-0716-1001-5_18

DOI: 10.1007/978-1-0716-1001-5

Kontakt

Institut für Veterinär-Anatomie

Koserstr. 20
14195 Berlin
+49 30 838 75784
anatomie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The intestine is often examined histologically in connection with allergies and in search for pathological changes. To be able to examine the intestine histologically with a microscope, it must be sampled and processed correctly. For microscopic analysis, the samples have to be cut into thin sections, stained, and mounted on slides. Since it is not possible to cut fresh samples without damaging them, they must first be fixed. The most common method, which is described herein, is the fixation in formalin with subsequent embedding in paraffin and staining of the slides with hematoxylin and eosin (H&E). Hematoxylin solutions (in this case Mayer’s hemalum solution) stain the acidic components of the cell, i.e., cell nuclei, blue. The staining with eosin gives a pink staining of cytoplasm. This chapter describes the method of processing intestinal tissue for paraffin-embedding, sectioning, and staining with H&E. Tissue processing can be done in tissue processing machines or manually. We describe the manual processing that is often used for smaller batches of samples.