Publication Database

Genetic diversity of Ornithobacterium rhinotracheale isolated from chickens and turkeys in the United States (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Nisar, Muhammad

Thieme, Susann (WE 8)

Hafez, Hafez M. (WE 15)

Sentíes-Cué, Gabriel

Chin, Richard P.

Muhammad, Sidra Pir

Aboubakr, Hamada

Goyal, Sagar M.

Nagaraja, Kakambi V.

Quelle

Avian diseases

Bandzählung: 64

Heftzählung: 3

Seiten: 324 – 329

ISSN: 0005-2086

Sprache

Englisch

Verweise

URL (Volltext): https://bioone.org/journals/avian-diseases/volume-64/issue-3/aviandiseases-D-20-00007/Genetic-Diversity-of-Ornithobacterium-rhinotracheale-Isolated-from-Chickens-and-Turkeys/10.1637/aviandiseases-D-20-00007.short

DOI: 10.1637/aviandiseases-D-20-00007

Kontakt

Institut für Lebensmittelsicherheit und -hygiene

Königsweg 69
14163 Berlin
+49 30 838 62551 / 52790
lebensmittelhygiene@vetmed.fu-berlin.de / fleischhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Ornithobacterium rhinotracheale (ORT) is an important bacterial pathogen of great economic significance to poultry production. This bacterium causes severe disease in chickens and turkeys worldwide. The objective of this study was to characterize ORT isolates from two different geographic locations in the U.S. by Multilocus Sequence Typing (MLST). A total of 60 isolates were included in this study; 36 from California and 24 from Minnesota. All 60 isolates were confirmed to be ORT by PCR that targeted the 16S rRNA gene. The results of MLST revealed eight different sequence types (STs) of ORT. Out of these four were novel and were assigned numbers ST-32, ST-33, ST-34 and ST-35. ST-1 was the predominant sequence type among all isolates followed by ST-9 and ST-8. Only one isolate was identified as ST-2. No significant variation was seen in sequence types in ORT isolated from different years. In turkeys, 76.3% (29/38) of isolates belonged to ST-1 and 7.9% (3/38) to ST-8. Of the chicken isolates, 72.2% (13/18) belonged to ST-1 and 16.6% (3/18) to ST-9. Isolates from both states showed low genetic variability. Of the 32 isolates from California, 24 (75%) were identified as ST-1 and 4 (12.5%) were identified as ST-9. The most prevalent sequence type was ST-1 (17/24), followed by ST-8 (3/24) in Minnesota. Three isolates from turkeys in Minnesota were identified as ST-8, which was closely related to sequence type (RefO) isolated from a rook in Germany in 2000. Whether this sequence type had evolved from wild birds could not be ascertained in this study.