Publikationsdatenbank

Combined methylome, transcriptome and proteome analyses document rapid acclimatization of a bacterium to environmental changes (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Srivastava, Abhishek

Murugaiyan, Jayaseelan (WE 10)

Garcia, Juan A. L.

De Corte, Daniele

Hoetzinger, Matthias

Eravci, Murat

Weise, Christoph

Kumar, Yadhu

Roesler, Uwe (WE 10)

Hahn, Martin W.

Grossart, Hans-Peter

Quelle

Frontiers in microbiology

Bandzählung: 11

Seiten: Article 544785

ISSN: 1664-302x

Sprache

Englisch

Verweise

URL (Volltext): https://www.frontiersin.org/articles/10.3389/fmicb.2020.544785/full

DOI: 10.3389/fmicb.2020.544785

Pubmed: 33042055

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Polynucleobacter asymbioticus strain QLW-P1DMWA-1T represents a group of highly successful heterotrophic ultramicrobacteria that is frequently very abundant (up to 70% of total bacterioplankton) in freshwater habitats across all seven continents. This strain was originally isolated from a shallow Alpine pond characterized by rapid changes in water temperature and elevated UV radiation due to its location at an altitude of 1300 m. To elucidate the strain's adjustment to fluctuating environmental conditions, we recorded changes occurring in its transcriptomic and proteomic profiles under contrasting experimental conditions by simulating thermal conditions in winter and summer as well as high UV irradiation. To analyze the potential connection between gene expression and regulation via methyl group modification of the genome, we also analyzed its methylome. The methylation pattern differed between the three treatments, pointing to its potential role in differential gene expression. An adaptive process due to evolutionary pressure in the genus was deduced by calculating the ratios of non-synonymous to synonymous substitution rates for 20 Polynucleobacter spp. genomes obtained from geographically diverse isolates. The results indicate purifying selection.