Publication Database

Bacteriophage amplification:
a comparison of selected methods (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Skaradzińska, Aneta

Ochocka, Marta

Śliwka, Paulina

Kuźmińska-Bajor, Marta

Skaradziński, Grzegorz

Friese, Anika (WE 10)

Roschanski, Nicole (WE 10)

Murugaiyan, Jayaseelan (WE 10)

Roesler, Uwe (WE 10)

Quelle

Journal of Virological Methods

Bandzählung: 282

Seiten: 113856

ISSN: 0166-0934

Sprache

Englisch

Verweise

URL (Volltext): https://www.sciencedirect.com/science/article/abs/pii/S0166093420301087

DOI: 10.1016/j.jviromet.2020.113856

Pubmed: 32198027

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The bactericidal properties of bacteriophages have been used almost since the moment of the discovery of bacterial viruses. In the light of the rapidly growing number of antibiotic-resistant bacteria, phage therapy is considered one of the most promising alternatives to classical treatment. Phage amplification is one of the most common procedures of working with phages, and high-titer preparations are beneficial at the experimental stage of studies as well as in practice. The objective of this study was to compare five commonly applied methods of phage amplification: (i) pooled plaques method, (ii) the plate wash method, (iii) the agar culture method, (iv) the two-stage culture method, and (v) in liquid culture. All methods were tested for fifteen different phages. The results described herein indicate that there is no optimal, universal method for phage amplification, and the most effective method has to be established individually for each phage.