Publikationsdatenbank

CD4+ Th immunogenicity of the Ascaris spp. secreted products (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Ebner, Friederike (WE 6)

Morrison, Eliot

Bertazzon, Miriam

Midha, Ankur (WE 6)

Hartmann, Susanne (WE 6)

Freund, Christian

Álvaro-Benito, Miguel

Quelle

npj vaccines

Bandzählung: 5

Seiten: Article number: 25

ISSN: 2059-0105

Sprache

Englisch

Verweise

URL (Volltext): https://www.nature.com/articles/s41541-020-0171-z

DOI: 10.1038/s41541-020-0171-z

Pubmed: 32218997

Kontakt

Institut für Immunologie

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51834
immunologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Ascaris spp. is a major health problem of humans and animals alike, and understanding the immunogenicity of its antigens is required for developing urgently needed vaccines. The parasite-secreted products represent the most relevant, yet complex (>250 proteins) antigens of Ascaris spp. as defining the pathogen-host interplay. We applied an in vitro antigen processing system coupled to quantitative proteomics to identify potential CD4+ Th cell epitopes in Ascaris-secreted products. This approach considerably restricts the theoretical list of epitopes using conventional CD4+ Th cell epitope prediction tools. We demonstrate the specificity and utility of our approach on two sets of candidate lists, allowing us identifying hits excluded by either one or both computational methods. More importantly, one of the candidates identified experimentally, clearly demonstrates the presence of pathogen-reactive T cells in healthy human individuals against these antigens. Thus, our work pipeline identifies the first human T cell epitope against Ascaris spp. and represents an easily adaptable platform for characterization of complex antigens, in particular for those pathogens that are not easily amenable for in vivo experimental validation.