Publication Database

Mechanisms of linezolid resistance among enterococci of clinical origin in Spain:
detection of optrA- and cfr(D)-carrying E.faecalis (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Ruiz-Ripa, Laura

Feßler, Andrea T. (WE 7)

Hanke, Dennis (WE 7)

Eichhorn, Inga (WE 7)

Azcona-Gutiérrez, José Manuel

Pérez-Moreno, Mar Olga

Seral, Cristina

Aspiroz, Carmen

Alonso, Carla Andrea

Torres, Luis

Alós, Juan-Ignacio

Schwarz, Stefan (WE 7)

Torres, Carmen

Quelle

Microorganisms : open access journal

Bandzählung: 8

Heftzählung: 8

Seiten: 1155

ISSN: 2076-2607

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/2076-2607/8/8/1155

DOI: 10.3390/microorganisms8081155

Pubmed: 32751552

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The mechanisms of linezolid resistance among 13 E. faecalis and 6 E. faecium isolates, recovered from six Spanish hospitals during 2017-2018, were investigated. The presence of acquired linezolid resistance genes and mutations in 23S rDNA and in genes encoding for ribosomal proteins was analyzed by PCR and amplicon sequencing. Moreover, the susceptibility to 18 antimicrobial agents was investigated, and the respective molecular background was elucidated by PCR-amplicon sequencing and whole genome sequencing. The transferability of the linezolid resistance genes was evaluated by filter-mating experiments. The optrA gene was detected in all 13 E. faecalis isolates; and one optrA-positive isolate also carried the recently described cfr(D) gene. Moreover, one E. faecalis isolate displayed the nucleotide mutation G2576T in the 23S rDNA. This mutation was also present in all six E. faecium isolates. All linezolid-resistant enterococci showed a multiresistance phenotype and harbored several antimicrobial resistance genes, as well as many virulence determinants. The fexA gene was located upstream of the optrA gene in 12 of the E. faecalis isolates. Moreover, an erm(A)-like gene was located downstream of optrA in two isolates recovered from the same hospital. The optrA gene was transferable in all but one E. faecalis isolates, in all cases along with the fexA gene. The cfr(D) gene was not transferable. The presence of optrA and mutations in the 23S rDNA are the main mechanisms of linezolid resistance among E. faecalis and E. faecium, respectively. We report the first description of the cfr(D) gene in E. faecalis. The presence of the optrA and cfr(D) genes in Spanish hospitals is a public health concern.