Publikationsdatenbank

Dietary supplementation of DL-Methionine potently induces sodium-dependent L-Methionine absorption in porcine jejunum ex vivo (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Romanet, Stella (WE 2)

Aschenbach, Jörg R. (WE 2)

Pieper, Robert (WE 4)

Zentek, Jürgen (WE 4)

Htoo, John H.

Whelan, Rose A.

Mastrototaro, Lucia (WE 2)

Quelle

The journal of nutrition

Bandzählung: 150

Heftzählung: 7

Seiten: 1782 – 1789

ISSN: 0022-3166

Sprache

Englisch

Verweise

URL (Volltext): https://academic.oup.com/jn/article/150/7/1782/5828191

DOI: 10.1093/jn/nxaa115

Pubmed: 32359147

Kontakt

Institut für Tierernährung

Königin-Luise-Str. 49
14195 Berlin
+49 30 838 52256
tierernaehrung@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background:
Methionine is an essential amino acid (AA) with many fundamental roles. Humans often supplement LMet, whereas DL-Met and DL-2-hydroxy-4-(methylthio)butanoic acid (DL-HMTBA) are more frequently used to supplement livestock.

Objectives:
The study aimed to investigate whether dietary Met source alters the absorptive capacity for Met isomers in the small intestine of piglets.

Methods:
A total of 27 male 10-wk-old piglets in 3 feeding groups received a diet supplemented with 0.21% DL-Met, 0.21% L-Met, or 0.31% DL-HMTBA to meet the Met + cystine requirement. After ≥10 d, absorptive fluxes of D-Met or L-Met were measured at a physiological concentration of 50 μM and a high concentration of 5 mM in duodenum, middle jejunum, and ileum ex vivo. Data were compared by 2-factor ANOVA.

Results:
Across diets, fluxes of both Met isomers at both tested concentrations increased from duodenum to ileum by a factor of ∼2–5.5 (P < 0.05). Pigs supplemented with DL-Met had greater (P < 0.085) absorptive fluxes at 50 μM L-Met (0.50, 2.07, and 3.86 nmol · cm−2 · h−1) and D-Met (0.62, 1.41, and 1.19 nmol · cm−2 · h−1) than did pigs supplemented with DL-HMTBA (L-Met: 0.28, 0.76, and 1.08 nmol · cm−2 · h−1; D-Met: 0.34, 0.58, and 0.64 nmol · cm−2 · h−1) in duodenum, jejunum, and ileum, respectively. Only in jejunum of DL-Met-fed pigs, fluxes at 50 μM L-Met were reduced by the omission of luminal Na+ (from 3.27 to 0.86 nmol · cm−2 · h−1; P < 0.05) and by a cocktail of 22 luminal AAs (to 1.05 nmol · cm−2 · h−1; P < 0.05).

Conclusions:
Dietary supplementation of DL-Met increases the efficiency of L-Met and D-Met absorption at physiologically relevant luminal Met concentrations along the small intestine of pigs, including a very prominent induction of an Na+-dependent transport system with preference for L-Met in the mid-jejunum. Dietary supplementation with DLMet could be a promising tool to improve the absorption of Met and other AAs.