Publikationsdatenbank

High frequency of benzimidazole resistance alleles in trichostrongyloids from Austrian sheep flocks in an alpine transhumance management system (2020)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Hinney, Barbara

Schoiswohl, Julia

Melville, Lynsey

Ameen, Vahel J. (WE 13)

Wille-Piazzai, Walpurga

Bauer, Karl

Joachim, Anja

Krücken, Jürgen (WE 13)

Skuce, Philip J.

Krametter-Frötscher, Reinhild

Quelle

BMC veterinary research

Bandzählung: 16

Heftzählung: 1

Seiten: Article number 132

ISSN: 1746-6148

Sprache

Englisch

Verweise

URL (Volltext): https://bmcvetres.biomedcentral.com/track/pdf/10.1186/s12917-020-02353-z

DOI: 10.1186/s12917-020-02353-z

Pubmed: 32393382

Kontakt

Institut für Parasitologie und Tropenveterinärmedizin

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 62310
parasitologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background:
Infections of small ruminants with trichostrongyloid nematodes often result in reduced productivity and may be detrimental to the host. Anthelmintic resistance (AR) against most anthelmintic drug classes is now widespread amongst the trichostrongyloids. Baseline establishment, followed by regular monitoring of the level of AR, is necessary for farmers and veterinarians to make informed decisions about parasite management. The detection of single nucleotide polymorphisms (SNPs) is a sensitive method to detect AR against benzimidazoles (BZs), one of the most widely used anthelmintic classes. Alpine transhumance constitutes a special type of pasturing of sheep from many different farms, the aim of this study was to investigate the prevalence of benzimidazole resistance alleles in this particular management system.

Results:
Sixteen sheep flocks in Styria and Salzburg in Austria were examined by pyrosequencing for SNPs at codons 167, 198 and 200 of the isotype-1 β-tubulin gene. The frequency of the resistance-associated exchange F200Y was 87–100% for H. contortus, 77–100% for T. colubriformis and <  5–66% for T. circumcincta. Additionally, the F167Y polymorphism was detected in T. colubriformis from two farms at a frequency of 19 and 23% respectively.

Conclusions:
The high resistance allele frequency in H. contortus and T. colubriformis in the examined sheep population urgently calls for the development of new treatment strategies to sustainably control trichostrongyloid infections for this kind of pasturing, since the frequent mixing of flocks during the alpine summer grazing must be considered an important risk factor for the spread of resistant nematodes to a large number of farms.