Publikationsdatenbank

Effects of wet spray fixation on cell quality of equine bronchoalveolar lavage and tracheal wash fluid (2019)

Art

Vortrag

Autoren

Bertram, C. A. (WE 12)

Dietert, K. (WE 12)

Pieper, L. (WE 16)

Kühnel, S.

Erickson, N. A. (WE 12)

Barton, A. K. (WE 17)

Klopfleisch, R. (WE 12)

Kongress

Joint Congress of Veterinary Pathology and Veterinary Clinical Pathology

Arnhem, 25. – 28.09.2019

Quelle

Veterinary clinical pathology

Bandzählung: 48

Heftzählung: 4

Seiten: 804

ISSN: 0275-6382

Sprache

Englisch

Verweise

URL (Volltext): https://onlinelibrary.wiley.com/doi/epdf/10.1111/vcp.12798

DOI: 10.1111/vcp.12798

Kontakt

Institut für Veterinär-Epidemiologie und Biometrie

Königsweg 67
14163 Berlin
+49 30 838 56034
epi@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background:
Knowledge of variables influencing cytological sample quality of equine bronchoalveolar lavage fluid (BALF) is of major rel-evance for reliable cytological interpretation.

Objective:
To examine the influence of on-slide, spray-fixation on cellular quality in comparison to samples prepared ordinarily without postfixation (solely air-dried).

Methods:
Bronchoalveolar lavage fluids of five horses were pro-cessed by cytospin preparation and tracheal washes (TW) of 10 horses were prepared by manual smearing. Specimens were fixed subsequently with a commercially available fixation spray (Roti- Fix spray; Carl Roth GmbH + Co KG, Karlsruhe, Germany) in a wet state or were solely air-dried. Three specimens per fluid collection method, horse, and fixation method were obtained and were stained with May-Grünwald Giemsa. In specimens of BALF, 500 cells within a standard template were scored semiquantitatively by a four-tier morphology score (MS3 = perfect cell morphology to MS0 = mor-phology completely lost). Cellular morphology of TW specimens was assessed by paired comparison between spray-fixed and air-dried samples.

Results:
Cytospins of BALF samples fixed with the fixation spray had a significantly (P = .013) superior cell morphology (mean MS: 2.227) in comparison to solely air-dried specimens (mean MS: 2.040). Similarly, the cell morphology of 84.6% of the TW samples was per-ceived to be superior with spray-fixation in paired comparison to solely air-dried samples.

Conclusion:
On-slide spray-fixation is a very simple method that may be easily conducted in veterinary clinics and ambulatory practices. Usage of the fixation-spray has led to improved preser-vation of cell morphology and is therefore recommended by the authors.