Publikationsdatenbank

Reptiles are relevant carriers of Clostridioides difficile (2019)

Art

Poster

Autoren

Rabold, Denise (WE 7)

Mundhenk, Lars (WE 12)

Seyboldt, Christian

Lübke-Becker, Antina (WE 7)

Kongress

11th International Conference on the Molecular Biology and Pathogenesis of the Clostridia (ClostPath)

Leiden, Niederlande, 19. – 22.08.2019

Quelle

CLOSTPATH 11 - Leiden, August 19-22, 2019 : Abstract book : Stadsgehoorzaal — 11th International Conference on the molecular biology and pathogenesis of the Clostridia (Hrsg.)

Leiden, The Netherlands: 11th International Conference on the molecular biology and pathogenesis of the Clostridia, 2019 — S. 175–176

Sprache

Englisch

Verweise

URL (Volltext): https://clostpath2019.org/wp-content/uploads/2019/08/Clostpath2019_Abstractbook.pdf

Kontakt

Institut für Tierpathologie

Robert-von-Ostertag-Str. 15
14163 Berlin
+49 30 838 62450
pathologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Clostridioides (previously Clostridium) difficile can cause human gastrointestinal infections ranging from mild diarrhoea to life-threatening pseudomembranous colitis that are often healthcare associated. Nevertheless, one quarter of the human C. difficile infections (CDI) is estimated to occur within the community (1) and a high overlap of ribotypes occurring in humans and various animal species has already been described (2) pointing to possible zoonotic transmission. reptiles are increasingly popular pets and there is virtually no information about their C. difficile carriage and burden of disease.
During November 2017 and March 2019, 48 faecal (n=13) and organ samples (n=35) were collected originating from 25 individuals belonging to 16 different reptile species of different origin. All samples were screened for the presence of C. difficile by direct plating and enrichment culturing according to A. Schneeberg et al. (3). All C. difficile strains will be characterized using capillary gel electrophoresis based PCr ribotyping and PCr detection of toxin genes A, b, and the binary toxin genes. Additionally, Multilocus VNTr Analysis (MLVA) and antimicrobial susceptibility testing will be performed.
C. difficile was isolated from 15 (29%) out of 51 samples originating from 12/25 (48%) tested individuals. Although molecular characterization has not yet been completed, preliminary results revealed some well known toxigenic ribotypes that have already previously been related to livestock, humans or the environment, e.g. ribotypes 002/2, 005, and 049. Clinical and pathological data suggest that C. difficile can be associated with fatal conditions in reptiles.
This is the first survey on C. difficile in various reptile species. The results presented here prove that isolation rates of C. difficile in reptiles are comparably high; hence, C. difficile has to be considered as a potential pathogen in reptiles. Furthermore, reptiles may represent a possible source of infection for CDI in humans.