Publikationsdatenbank

Isolation and characterization of methicillin-resistant Staphylococcus pseudintermedius from ocular surfaces of dogs and cats suffering from ophthalmological diseases (2019)

Art

Vortrag

Autoren

Soimala, Tanawan (WE 20)

Lübke-Becker, Antina (WE 7)

Schwarz, Stefan (WE 7)

Eule, Johanna Corinna (WE 20)

Kongress

8th Symposium on Antimicrobial Resistance in Animals and the Environment

Tours, France, 01. – 03.06.2019

Quelle

Abstracts book : 8th Symposium on Antimicrobial Resistance in Animals and the Environment : 2019 July 1-3, Tours, France — UMR 1282 Infectiologie et Santé PubliqueInstitut National de la Recherche Agronomique (Hrsg.)

Tours, France: INRA Science & Impact, 2019 — S. 28

Sprache

Englisch

Verweise

URL (Volltext): https://symposium.inrae.fr/arae2019/content/download/4282/45774/version/1/file/ARAE2019_Abstract_Book_VF.pdf

Kontakt

Institut für Mikrobiologie und Tierseuchen

Robert-von-Ostertag-Str. 7-13
14163 Berlin
+49 30 838 51843 / 66949
mikrobiologie@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background and objectives:
Staphylococcus pseudintermedius isolates represent the leading cause of skin, ear, and postoperative bacterial infections in dogs. Methicillin-resistant S. pseudintermedius (MRSP) isolates have recently emerged as a major therapeutic challenge because of their antimicrobial multidrug resistance and their role as nosocomial pathogen. Up to now, the information regarding MRSP on ocular surfaces of dogs and cats in Germany is scarce.
Therefore, the purposes of this study were (i) to determine the prevalence of MRSP in conjunctival swabs from dogs and cats in relation to different types of ocular diseases and (ii) to investigate the MRSP isolates for their antimicrobial resistance phenotypes.

Materials and methods:
Conjunctival swabs were collected from 72 dogs and 24 cats. The samples were categorized according to the underlying disease into three different groups (group 1: conjunctivitis/blepharitis, n=31; group 2: keratitis, n=27; group 3: uveitis, n=38). Presumable S. pseudintermedius colonies were verified by MALDI TOF MS and/or VITEK®2 system. The determination of minimal inhibitory concentrations (MIC values) of antimicrobial agents was performed by broth microdilution according to the recommendations given in the CLSI document VET08 (2018). All phenotypically oxacillin-resistant S. pseudintermedius isolates were investigated by PCR for the methicillin resistance genes mecA and mecC.

Results:
S. pseudintermedius was isolated from 38/96 (39.5%) swabs. In total, 3/38 (8.1%) of the S. pseudintermedius isolates were confirmed as MRSP resulting in an overall prevalence of 3.1 % for MRSP. All three isolates originated from dogs and harbored the gene mecA. One of the three MRSP isolates (IMT 515/16) originated from a case of uveitis while the remaining two MRSP isolates (IMT 360/16 and IMT1670/16) were from cases of conjunctivitis/blepharitis. All three MRSP isolates exhibited a multidrug resistance phenotype, i.e. resistance to three and more classes of antimicrobial agents. The two MRSP isolates IMT 360/16 and IMT 515/16 showed the same resistance phenotypes, including resistance to erythromycin, clindamycin, streptomycin, gentamicin, trimethoprim/sulfamethoxazole, tetracycline, doxycycline, and enrofloxacin. The remaining MRSP isolate exhibited a slightly different resistance pattern, which included resistance to streptomycin, enrofloxacin, tetracycline, doxycycline, and trimethoprim/sulfamethoxazole. All three MRSP isolates were susceptible to linezolid, vancomycin, quinupristin/dalfopristin, florfenicol, and tiamulin.

Conclusion:
The study demonstrated a low prevalence of MRSP on the ocular surfaces of canine and feline ophthalmic patients. Due to their expanded antimicrobial resistance, it is strongly recommended to test MRSP isolates for their susceptibility to antimicrobial agents before using antimicrobial agents for therapeutic interventions.