Königsweg 67
14163 Berlin
+49 30 838 56034
epi@vetmed.fu-berlin.de
The objective of this observational experiment was to determine the association between the interval from reaching activity threshold (AT) using an automated activity monitoring system and the time of artificial insemination (AI) on pregnancy per AI (P/AI) for lactating Holstein cows inseminated with either fresh or frozen semen. Lactating Holstein cows (n = 3,420) from 7 commercial dairy farms were inseminated based on the alert of an accelerometer system
(Heatime; SCR Engineers Ltd., Netanya, Israel). Pregnancy diagnosis was performed by transrectal palpation 35 ± 7 d after AI. Data were analyzed using the GENLINMIXED procedure in SPSS. Estrus intensity was categorized based on peak activity into high (index value 35 to 80), medium (81 to 99), and high intensity estrus (100). The mean (± standard error of the mean) interval from onset of estrus (index value ≥ 35) to AI was 15.0 ± 0.34 hours.
Pregnancy per AI was affected by parity (P = 0.001), season (P = 0.001), estrus intensity (P = 0.001), and the interval from reaching AT to AI (P = 0.001). Type of semen (P = 0.231) had no effect on P/AI. Primiparous cows (31.1 %) had higher P/AI compared with multiparous cows (24.9 %). Cows inseminated in the hot season (23.7 %) had lower P/AI compared with the cold season (32.4 %). Cows with a high intensity estrus (32.7 %) were more fertile
compared with medium (29.1 %) or low intensity estrus (22.4 %). There was a quadratic effect of the interval from reaching AT and AI on probabilities of pregnancy. Pregnancy per AI at 35 d after AI was highest for cows inseminated between 13 to 24 hours after onset of estrus (-24 - 0 hours = 22.7%, 1 - 6 hours = 23.0%, 7 - 12 hours = 30.0%, 13 - 18 hours = 34.0%, 19 - 24 hours = 32.0%, 25 - 48 hours = 26.8%). In conclusion, inseminating cows 13 to 24 hours after passing the AT yielded the highest P/AI irrespective of type of semen whereas inseminating cows before or 6 h after reaching AT was detrimental on P/AI. A high intensity estrus was favorable for P/AI.