Publikationsdatenbank

BMP2 and TGF-ß cooperate differently during synovial-derived stem-cell Chondrogenesis in a Dexamethasone-dependent manner (2019)

Art

Zeitschriftenartikel / wissenschaftlicher Beitrag

Autoren

Kovermann, Nikolas J. (WE 17)

Basoli, Valentina

Della Bella, Elena

Alini, Mauro

Lischer, Christoph (WE 17)

Schmal, Hagen

Kubosch, Eva Johanna

Stoddart, Martin J.

Quelle

Cells : open access journal

Bandzählung: 8

Heftzählung: 6

Seiten: Article 636

ISSN: 2073-4409

Sprache

Englisch

Verweise

URL (Volltext): https://www.mdpi.com/2073-4409/8/6/636

DOI: 10.3390/cells8060636

Pubmed: 31242641

Kontakt

Pferdeklinik

Oertzenweg 19 b
14163 Berlin
+49 30 838 62299 / 62300
pferdeklinik@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Recent studies highlighting mesenchymal stem cell (MSC) epigenetic memory suggest that a different differentiation medium may be required depending on the tissue of origin. As synovial-derived stem cells (SDSCs) attract interest we aimed to investigate the influence of TGF-β1, BMP-2 and dexamethasone on SDSC chondrogenesis in vitro. We demonstrate that dexamethasone-free medium led to enhanced chondrogenic differentiation at both the mRNA and matrix level. The greatest COL2A1/COL10A1 ratio was detected in cells exposed to a combination medium containing 10 ng/mL BMP-2 and 1 ng/mL TGF-β1 in the absence of dexamethasone, and this was reflected in the total amount of glycosaminoglycans produced. In summary, dexamethasone-free medium containing BMP-2 and TGF-β1 may be the most suitable when using SDSCs for cartilage tissue regeneration.