Publikationsdatenbank

Inactivation of ESBL/AmpC-producing Escherichia coli in chicken manure during aeration and anaerobic digestion (2019)

Art

Vortrag

Autoren

Thomas, Corinna

Idler, Christine

Ammon, Christian

Herrmann, Christiane

Amon, Thomas (WE 10)

Kongress

XIX International Congress on Animal Hygiene 2019

Wroclaw, Poland, 08. – 12.09.2019

Quelle

Proceedings of the XIXth International Congress of the International Society for Animal Hygiene : Animal hygiene as a fundament of one health and welfare improving biosecurity, environment and food quality — International Society for Animal Hygiene (Hrsg.)

Wrocław, Poland: University of Environmental and Life Sciences, 2019 — S. 87

Sprache

Englisch

Verweise

URL (Volltext): https://www.isah-soc.org/userfiles/downloads/proceedings/2019_Proceedings.pdf

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

The high prevalence of extended-spectrum-ß-lactamase (ESBL)-producing Escherichia (E.) coli in European broiler farms leads to the possible dissemination of antibiotic-resistant strains into the environment when using contaminated feces as organic fertilizer. The aim of the present study was to determine the effects of aerobic and anaerobic mesophilic treatments on the survival of two artificially added ESBL/AmpC-producing E. coli strains during lab-scale aeration and anaerobic digestion of chicken manure.Two-liter-reactors were filled with chicken manure and either water for the aeration trials or digestate for the anaerobic digestion to get initial dry matter contents below 5 %. All reactors were then spiked with either an ESBL-producing or an AmpC-producing E. coli strain, both isolated from chicken feces. The reactors were then incubated aerobically or anaerobically at 37 °C and 42 °C, and the number of E. coli was determined over a period of 35 days. In addition, all samples were analyzed for total ammoniacal nitrogen, volatile fatty acids (VFA) and pH.Starting with initial levels of 107 colony forming units (cfu) per milliliter, both E. coli strains were below the detection limit (< 100 cfu/ml) after 35 days for both treatments and temperatures. At 37 °C, E. coli reduction was faster during aeration than during anaerobic digestion, while at 42 °C, there was no significant difference between treatments. Both 37 and 42 °C led to similar reduction kinetics during aeration while the 5 °C-difference led to a significantly faster reduction during anaerobic digestion. During aeration, the pH was significantly lower and the concentrations of VFA higher than during anaerobic digestion.Aeration and anaerobic digestion of chicken manure are suitable treatments to reduce antibiotic-resistant E. coli in chicken manure. The results may help define time-temperature guidelines for biogas plants and aeration may be a potential solution for wastewater treatment.