Publikationsdatenbank

Survival and transmission dynamics of Campylobacter spp. in broiler farm surroundings (2019)

Art

Poster

Autoren

Reichelt, B. (WE 10)

Szott, V. (WE 10)

Friese, A. (WE 10)

Roesler, U. (WE 10)

Kongress

Zoonoses 2019 - International Symposium on Zoonoses Research

16. – 18.10.2019

Quelle

Zoonoses 2019 - International Symposium on Zoonoses Research : Book of Abstracts — International Symposium on Zoonoses Research (Hrsg.)

Berlin, 2019 — S. 140

Sprache

Englisch

Verweise

URL (Volltext): https://evis.events/event/79/attachments/23/154/Book_of_Abstracts_Zoonoses2019.pdf

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Background and objectives:
Persistence in the environment as well as the viable but non-culturable (VBNC) state of Campylobacter spp. still remains an under explored field. We investigate possible directions of transmissions and reservoirs of Campylobacter spp. in the environment of broiler farms. Therefore, Campylobacter-positive broiler farms and their environment are sampled intensively. Campylobacter cultivation is done according to DIN ISO 10272. Isolates are species typed with MALDI-ToF analyses. To selectisolatesfor whole genome sequencingflaA typesare determined.

Results:
We observed high prevalence of Campylobacter spp. in the investigated barn samples (57%) during summer. In comparison we observed lower amounts during winter (32,5 %). First evidence of cultivable Campylobacter spp. in the environmentwas detected in water-associated matrices in Summer. During winter Campylobacter could be isolated from different environmental sources. Campylobacter was not cultivable after cleaning and disinfection. Most of the analysed samples were identified as C. jejuni. FlaA-typing revealed predominant flaA typesthat changed aftercleaning and disinfection.

Conclusion:
Traces of cultivable Campylobacter in the environment show that there is the necessity for deeper investigations especially the (VBNC) state. Therefore, we plan to include a live/dead discriminatory qPCR methodaiming to detect possible VBNC state Campylobacter in this ongoing project.