Publikationsdatenbank

Emissions of Campylobacter spp. from broiler farms and persistence in their environment (2019)

Art

Vortrag

Autoren

Reichelt, Benjamin (WE 10)

Szott, Vanessa (WE 10)

Friese, Katrin Anika (WE 10)

Roesler, Uwe (WE 10)

Kongress

XIX International Congress of ISAH 2019

08. – 12.09.2019

Quelle

Proceedings of the XIXth International Congress of the International Society for Animal Hygiene : Animal hygiene as a fundament of one health and welfare improving biosecurity, environment and food quality — International Society for Animal Hygiene (Hrsg.)

Wrocław, Poland: University of Environmental and Life Sciences, 2019 — S. 153

Sprache

Englisch

Verweise

URL (Volltext): https://www.isah-soc.org/?Events/Congresses/2019-Poland

Kontakt

Institut für Tier- und Umwelthygiene

Robert-von-Ostertag-Str. 7-13
14169 Berlin
+49 30 838 51845
tierhygiene@vetmed.fu-berlin.de

Abstract / Zusammenfassung

Occurrence of Campylobacter spp. in broiler farms and their environment is considered a major public health and
economical concern.

Persistence and survivability in the environment as well as the viable but non-culturable (VBNC) state of Campylobacter spp. are not completely researched yet. Therefore, we carry out a largescale investigation aiming towards the detection of possible emission pathways and reservoirs of persistent Campylobacter spp. in the environment.
Campylobacter-positive tested broiler farms and their environment are sampled intensively by taking pooled faeces, boot swabs, air, water and swab samples from various surfaces and insects. Two consecutive fattening periods are investigated at the end of the fattening period, each. In addition, we also assess the efficacy of the cleaning and disinfection procedure in between the two fattening periods. To differentiate between environmental conditions all farms are sampled in summer and winter. Samples are processed according to DIN ISO 10272 for Campylobacter spp.. Afterwards positive isolates are species typed with MALDI-ToF analyses and multiplex PCR assay. Flagellin genes (flaA) of Campylobacter spp. are determined to select isolates for whole-genome sequencing.
We observed high prevalence of Campylobacter spp. in the investigated barn samples (67%) in this ongoing project. After cleaning and disinfection there was no Campylobacter spp. detectable. In the environment one sample was tested positive. This showed first evidence of cultivable Campylobacter spp. in a water-associated matrix. All analysed samples were identified as C. jejuni and FlaA- typing revealed two predominant flaA types (36, 57). Further results of the ongoing project will be presented.
Our first summer investigation results showed almost no emissions of cultivable Campylobacter spp. from the broiler farms. Further investigations will include the winter term investigations and the detection of the VBNC state using propidium monoazide quantitative PCR (PMAqPCR).